Background: Genomic and transcriptomic approaches have the potential for unveiling the genome-wide response to environmental perturbations. The abundance of the catadromous European eel (Anguilla anguilla) stock has been declining since the 1980s probably due to a combination of anthropogenic and climatic factors. In this paper, we explore the transcriptomic dynamics between individuals from high (river Tiber, Italy) and low pollution (lake Bolsena, Italy) environments, which were measured for 36 PCBs, several organochlorine pesticides and brominated flame retardants and nine metals. Results: To this end, we first (i) updated the European eel transcriptome using deep sequencing data with a total of 640,040 reads assembled into 44,896 contigs (Eeelbase release 2.0), and (ii) developed a transcriptomic platform for global gene expression profiling in the critically endangered European eel of about 15,000 annotated contigs, which was applied to detect differentially expressed genes between polluted sites. Several detoxification genes related to metabolism of pollutants were upregulated in the highly polluted site, including genes that take part in phase I of the xenobiotic metabolism (CYP3A), phase II (glutathione-S-transferase) and oxidative stress (glutathione peroxidase). In addition, key genes in the mitochondrial respiratory chain and oxidative phosphorylation were down-regulated at the Tiber site relative to the Bolsena site. Conclusions: Together with the induced high expression of detoxification genes, the suggested lowered expression of genes supposedly involved in metabolism suggests that pollution may also be associated with decreased respiratory and energy production.

Pujolar, J., Marino, I., Milan, M., Coppe, A., Maes, G., Capoccioni, F., et al. (2012). Surviving in a toxic world: transcriptomics and gene expression profiling in response to environmental pollution in the critically endangered European eel. BMC GENOMICS, 13, 507-528 [10.1186/1471-2164-13-507].

Surviving in a toxic world: transcriptomics and gene expression profiling in response to environmental pollution in the critically endangered European eel

CAPOCCIONI, FABRIZIO;CICCOTTI, ELEONORA;
2012-09-25

Abstract

Background: Genomic and transcriptomic approaches have the potential for unveiling the genome-wide response to environmental perturbations. The abundance of the catadromous European eel (Anguilla anguilla) stock has been declining since the 1980s probably due to a combination of anthropogenic and climatic factors. In this paper, we explore the transcriptomic dynamics between individuals from high (river Tiber, Italy) and low pollution (lake Bolsena, Italy) environments, which were measured for 36 PCBs, several organochlorine pesticides and brominated flame retardants and nine metals. Results: To this end, we first (i) updated the European eel transcriptome using deep sequencing data with a total of 640,040 reads assembled into 44,896 contigs (Eeelbase release 2.0), and (ii) developed a transcriptomic platform for global gene expression profiling in the critically endangered European eel of about 15,000 annotated contigs, which was applied to detect differentially expressed genes between polluted sites. Several detoxification genes related to metabolism of pollutants were upregulated in the highly polluted site, including genes that take part in phase I of the xenobiotic metabolism (CYP3A), phase II (glutathione-S-transferase) and oxidative stress (glutathione peroxidase). In addition, key genes in the mitochondrial respiratory chain and oxidative phosphorylation were down-regulated at the Tiber site relative to the Bolsena site. Conclusions: Together with the induced high expression of detoxification genes, the suggested lowered expression of genes supposedly involved in metabolism suggests that pollution may also be associated with decreased respiratory and energy production.
25-set-2012
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore BIO/07 - ECOLOGIA
Settore BIO/18 - GENETICA
English
Con Impact Factor ISI
Anguilla anguilla; European eel; Transcriptome; Microarrays; Pollution
SCOPUS Indexed keywords EMTREE drug terms: cytochrome P450 3 Aglutathione peroxidase glutathione transferase EMTREE medical terms: article controlled study CYP3A gene detoxification down regulation eel endangered species gene expression gene expression profiling glutathione peroxidase geneglutathione S transferase gene Italy mitochondrial respiration non human nucleotide sequence oxidative phosphorylation oxidative stress pollutant pollution transcriptomic supregulation xenobiotic metabolism MeSH: Amino Acid Sequence Animals Cytochrome P-450 CYP3A Eels Electron Transpor tEndangered Species Environmental Pollution Flame Retardants Gene Expression Gene Expression Profiling Gene Expression Regulation Genome-Wide Association Study Glutathione Peroxidase Glutathione Transferase High-Throughput Nucleotide Sequencing Italy Molecular Sequence Data Oxidative Phosphorylation Pesticides Transcriptome Water Pollutants, Chemical Species Index: Anguilla anguilla
WOS Key Words Plus:ANGUILLA-ANGUILLA L; SARGASSO SEA; ECOLOGICAL GENOMICS; RIVER TIBER; X-RECEPTOR; POPULATIONS; WATER; RECRUITMENT; FIELD; METALLOTHIONEIN
Research Areas:Biotechnology & Applied Microbiology; Genetics & Heredity Web of Science Categories:Biotechnology & Applied Microbiology; Genetics & Heredity Metrics in Scopus 37 Citations 93rd Percentile 2.39 Field-Weighted Citation Impact 62 Mendeley Readers 94th Percentile WOS Metrics All Times Cited Counts 33 in All Databases 33 in Web of Science Core Collection 33 in BIOSIS Citation Index BMC Genomics has an Impact Factor of 3.729
Pujolar, J., Marino, I., Milan, M., Coppe, A., Maes, G., Capoccioni, F., et al. (2012). Surviving in a toxic world: transcriptomics and gene expression profiling in response to environmental pollution in the critically endangered European eel. BMC GENOMICS, 13, 507-528 [10.1186/1471-2164-13-507].
Pujolar, J; Marino, I; Milan, M; Coppe, A; Maes, G; Capoccioni, F; Ciccotti, E; Bervoets, L; Covaci, A; Belpaire, C; Cramb, G; Patarnello, T; Bargello...espandi
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/79260
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