The transcription factor p73 belongs to the p53 family of tumour suppressors and similar to other family members, transcribed as different isoforms with opposing pro- and anti-apoptotic functions. Unlike p53, p73 mutations are extremely rare in cancers. Instead, the pro-apoptotic activities of transcriptionally active p73 isoforms are commonly inhibited by over-expression of the dominant negative p73 isoforms. Therefore the relative ratio of different p73 isoforms is critical for the cellular response to a chemotherapeutic agent. Here, we analysed the expression of N-terminal p73 isoforms in cell lines and mouse tissues. Our data showed that the transcriptionally competent TAp73 isoform is abundantly expressed in cancer cell lines compared to the dominant negative ΔNp73 isoform. Interestingly, we detected higher levels of ΔNp73 in some mouse tissues, suggesting that ΔNp73 may have a physiological role in these tissues.

Conforti, F., Yang, A., Agostini, M., Rufini, A., Tucci, P., Nicklison Chirou, M., et al. (2012). Relative expression of TAp73 and ΔNp73 isoforms. AGING, 4(3), 202-205.

Relative expression of TAp73 and ΔNp73 isoforms

AGOSTINI, MASSIMILIANO;MELINO, GENNARO;
2012-03-01

Abstract

The transcription factor p73 belongs to the p53 family of tumour suppressors and similar to other family members, transcribed as different isoforms with opposing pro- and anti-apoptotic functions. Unlike p53, p73 mutations are extremely rare in cancers. Instead, the pro-apoptotic activities of transcriptionally active p73 isoforms are commonly inhibited by over-expression of the dominant negative p73 isoforms. Therefore the relative ratio of different p73 isoforms is critical for the cellular response to a chemotherapeutic agent. Here, we analysed the expression of N-terminal p73 isoforms in cell lines and mouse tissues. Our data showed that the transcriptionally competent TAp73 isoform is abundantly expressed in cancer cell lines compared to the dominant negative ΔNp73 isoform. Interestingly, we detected higher levels of ΔNp73 in some mouse tissues, suggesting that ΔNp73 may have a physiological role in these tissues.
mar-2012
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore BIO/11 - BIOLOGIA MOLECOLARE
English
Animals; HeLa Cells; DNA-Binding Proteins; HEK293 Cells; Humans; Transcription, Genetic; Mice; HCT116 Cells; Protein Isoforms; Tumor Suppressor Proteins; RNA, Messenger; Hep G2 Cells; Nuclear Proteins; Transfection; Alternative Splicing; Gene Expression Regulation; RNA Interference
Conforti, F., Yang, A., Agostini, M., Rufini, A., Tucci, P., Nicklison Chirou, M., et al. (2012). Relative expression of TAp73 and ΔNp73 isoforms. AGING, 4(3), 202-205.
Conforti, F; Yang, A; Agostini, M; Rufini, A; Tucci, P; Nicklison Chirou, M; Grespi, F; Velletri, T; Knight, R; Melino, G; Sayan, B
Articolo su rivista
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/78443
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