A reversed-phase high-performance liquid chromatographic method for the detection of boronophenylalanine is described. Determination was obtained by precolumn reaction of o-phthalaldehyde with a mixture of standard amino acids containing boronophenylalanine and separating the corresponding o-phthalaldehyde derivatives, using a Kromasil C-18, 250 x 4.6 mm, 5-μm particle size column, a step gradient with two buffers, a flow rate of 1.2 ml/min, a column temperature of 23°C, and fluorimetric detection (excitation and emission wavelengths of 330 and 430 nm, respectively). The use of such a method for assaying boronophenylalanine in biological samples was tested in neutralized perchloric acid blood and cerebral tissue extracts of rats treated with intracarotid administration of 300 mg/kg of body weight boronophenylalanine. Results of these experiments showed that the present HPLC method represents a valid alternative to currently available analytical techniques for assaying boronophenylalanine based on boron determination in terms of reproducibility, recovery, or sensitivity. Therefore, it is suggested that the present method may routinely be used in all preclinical and clinical studies in which quantification of circulating and tissue concentrations of boronophenylalanine is critical for the application of boron neutron capture therapy.

DI PIERRO, D., Lazzarino, G., Pastore, F.s., Tavazzi, B., DEL BOLGIA, F., Amorini, A., et al. (2000). Determination of boronophenylalanine in biological samples using precolumn o-phthalaldehyde derivatization and reversed-phase high-performance liquid chromatography. ANALYTICAL BIOCHEMISTRY, 284(2), 301-306 [10.1006/abio.2000.4715].

Determination of boronophenylalanine in biological samples using precolumn o-phthalaldehyde derivatization and reversed-phase high-performance liquid chromatography

DI PIERRO, DONATO;PASTORE, FRANCESCO SAVERIO;TAVAZZI, BARBARA;DEL BOLGIA, FRANCO;
2000-09-01

Abstract

A reversed-phase high-performance liquid chromatographic method for the detection of boronophenylalanine is described. Determination was obtained by precolumn reaction of o-phthalaldehyde with a mixture of standard amino acids containing boronophenylalanine and separating the corresponding o-phthalaldehyde derivatives, using a Kromasil C-18, 250 x 4.6 mm, 5-μm particle size column, a step gradient with two buffers, a flow rate of 1.2 ml/min, a column temperature of 23°C, and fluorimetric detection (excitation and emission wavelengths of 330 and 430 nm, respectively). The use of such a method for assaying boronophenylalanine in biological samples was tested in neutralized perchloric acid blood and cerebral tissue extracts of rats treated with intracarotid administration of 300 mg/kg of body weight boronophenylalanine. Results of these experiments showed that the present HPLC method represents a valid alternative to currently available analytical techniques for assaying boronophenylalanine based on boron determination in terms of reproducibility, recovery, or sensitivity. Therefore, it is suggested that the present method may routinely be used in all preclinical and clinical studies in which quantification of circulating and tissue concentrations of boronophenylalanine is critical for the application of boron neutron capture therapy.
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore MED/27 - Neurochirurgia
English
Boron neutron capture therapy; Boronophenylalanine; Brain tumors; Cerebral tissue; HPLC
DI PIERRO, D., Lazzarino, G., Pastore, F.s., Tavazzi, B., DEL BOLGIA, F., Amorini, A., et al. (2000). Determination of boronophenylalanine in biological samples using precolumn o-phthalaldehyde derivatization and reversed-phase high-performance liquid chromatography. ANALYTICAL BIOCHEMISTRY, 284(2), 301-306 [10.1006/abio.2000.4715].
DI PIERRO, D; Lazzarino, G; Pastore, Fs; Tavazzi, B; DEL BOLGIA, F; Amorini, A; Fazzina, G; Giuffrè, R
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/70951
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