The human glioblastoma cell line LI showed morphological features typical of its neuroectodermal origin. Cells were positive by immunofluorescence to GFAP, MHC class II, and L1 determinants. Cytogenetic analysis showed the presence of a modal chromosome number of 63, ranging from 58 to 69 chromosomes (DNA index was 1.6). Northern blot analysis demonstrated the presence of mRNA transcripts specific for transglutaminase C (type II or "tissue"), growth-hormone releasing-hormone (GHRH), insulin-like growth factor II (IGF-II), and proopiomelanocortin (POMC). The GHRH mRNA was present in two different sizes, one similar to the normal hypothalamic species of 0.75 kb, whilst the second species was a large transcript of approximately 10 kb size. Treatment with 5 microM retinoic acid or 5 mM alpha-difluoromethylornithine for 5 days sharply reduced the growth rate and also induced modulation of the ultrastructure and antigenic profile. This cell line may be useful to study glial differentiation and the relationship of GHRH, IGF-II and POMC expression with differentiation in neuroectodermal tumours.

Savarese, A., Annicchiarico Petruzzelli, M., Citro, G., Zupi, G., Spagnoli, L.g., Colantoni, A., et al. (1992). Characterisation of a human glioblastoma cell line (LI) expressing hypothalamic and pituitary hormones. EXPERIMENTAL BRAIN RESEARCH, 89(2), 408-414.

Characterisation of a human glioblastoma cell line (LI) expressing hypothalamic and pituitary hormones

SPAGNOLI, LUIGI GIUSTO;COLANTONI, ALFREDO;VERNOLE, PATRIZIA;GUERRIERI, PIETRO
1992-01-01

Abstract

The human glioblastoma cell line LI showed morphological features typical of its neuroectodermal origin. Cells were positive by immunofluorescence to GFAP, MHC class II, and L1 determinants. Cytogenetic analysis showed the presence of a modal chromosome number of 63, ranging from 58 to 69 chromosomes (DNA index was 1.6). Northern blot analysis demonstrated the presence of mRNA transcripts specific for transglutaminase C (type II or "tissue"), growth-hormone releasing-hormone (GHRH), insulin-like growth factor II (IGF-II), and proopiomelanocortin (POMC). The GHRH mRNA was present in two different sizes, one similar to the normal hypothalamic species of 0.75 kb, whilst the second species was a large transcript of approximately 10 kb size. Treatment with 5 microM retinoic acid or 5 mM alpha-difluoromethylornithine for 5 days sharply reduced the growth rate and also induced modulation of the ultrastructure and antigenic profile. This cell line may be useful to study glial differentiation and the relationship of GHRH, IGF-II and POMC expression with differentiation in neuroectodermal tumours.
1992
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore MED/08 - ANATOMIA PATOLOGICA
English
Hypothalamus; Blotting, Northern; Humans; Hormones; Pituitary Hormones; DNA, Neoplasm; Phenotype; Tumor Cells, Cultured; Tretinoin; Pro-Opiomelanocortin; Microscopy, Electron; Middle Aged; Glioma; Insulin-Like Growth Factor II; Growth Hormone-Releasing Hormone; Male; Nervous System Neoplasms; Cytogenetics
Savarese, A., Annicchiarico Petruzzelli, M., Citro, G., Zupi, G., Spagnoli, L.g., Colantoni, A., et al. (1992). Characterisation of a human glioblastoma cell line (LI) expressing hypothalamic and pituitary hormones. EXPERIMENTAL BRAIN RESEARCH, 89(2), 408-414.
Savarese, A; Annicchiarico Petruzzelli, M; Citro, G; Zupi, G; Spagnoli, Lg; Colantoni, A; Vernole, P; Stephanou, A; Knight, R; Guerrieri, P
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/69335
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