Rationale An abnormal essential fatty acid (EFAs) metabolism and a higher unsaturated trans FA (TFA) levels have been associated with allergy suggesting a link between fatty acid (FA) structures and atopic diseases. The aim of the present study was to correlate the atopic status with FA profile and TFA presence in cell membrane. Methods We studied the erythrocyte and T lymphocyte membranes obtained from 10 children affected by atopic dermatitis (AD). Total phospholipid fractions were analyzed by an innovative protocol made of two parallel gas chromatography (GC) analysis which include the cis/trans isomer identification of FA. Results In all patients the saturated/monounsaturated FA (SFA/MUFA) ratio was higher than normal range (1.7-2). The percentage of arachidonate 20:4 FA was normal but, in 3 patients, it was replaced by 20:3. The GC profile of C20 FA, compared with the profile of arachidonate isomerization, showed the presence of trans isomers at high levels. Moreover, trans isomer of oleic acid residues was also found in 50% of the patients and 20:5 (EPA) and 22:6 (DHA) FA were very low or absent in all the patients. Conclusions In erythrocyte and T cell membranes of our AD children a serious impairment of lipid metabolism was observed, consistent with a reduced rate of activity of delta 6 desaturase. The altered SFA/MUFA ratio together with the higher percentage of the trans isomeric forms might affect membrane structure or fluidity and consequently lead to an alteration of the immune receptor complexes within the lipid microdomains (rafts).
Chini, L., Angelini, F., Moschese, V., Dellonte, S., Chatgilialoglu, C., Iannini, R., et al. (2004). Altered lipid cell membrane profile in children affected by atopic dermatitis. JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 113(2, Supplement), S56-S56 -.
|Tipologia:||Articolo su rivista|
|Citazione:||Chini, L., Angelini, F., Moschese, V., Dellonte, S., Chatgilialoglu, C., Iannini, R., et al. (2004). Altered lipid cell membrane profile in children affected by atopic dermatitis. JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 113(2, Supplement), S56-S56 -.|
|IF:||Con Impact Factor ISI|
|Settore Scientifico Disciplinare:||Settore MED/38 - Pediatria Generale e Specialistica|
|Revisione (peer review):||Sì, ma tipo non specificato|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1016/j.jaci.2003.12.168|
|Stato di pubblicazione:||Pubblicato|
|Data di pubblicazione:||2004|
|Titolo:||Altered lipid cell membrane profile in children affected by atopic dermatitis|
|Autori:||Chini, L; Angelini, F; Moschese, V; Dellonte, S; Chatgilialoglu, C; Iannini, R; Polito, A; Rossi, P; Ferreri, C|
|Appare nelle tipologie:||01 - Articolo su rivista|