The human T-lymphotropic virus type I (HTLV-I) has been recently associated with cases of tropical spastic paraparesis and human myelopathy. In order to study whether cells of neuroectodermic origin were susceptible to HTLV-I infection, a human glioma cell line T67 was exposed in vitro to HTLV-I by a cell-free method of virus transmission. The presence of HTLV-I proviral DNA was analyzed by polymerase chain reaction 3, 7, and 14 days after infection. The results showed the presence of LTR, pol, and tax sequences within glioma cell line 3 days after the infection. However after 7 and 14 days, detection of HTLV-I sequences remarkably decreased. P19 expression peaked 7 days after infection and decreased in the following week. These data provide evidence that cell-free transmission of HTLV-I results in transient infection of cells of glial origin.

Graziani, G., Faraoni, I., Zhang, J., Caronti, B., Lauro, G., Bonmassar, E., et al. (1993). Transient HTLV-I infection of a human glioma cell line following cell-free exposure. VIROLOGY, 197(2), 767-769 [10.1006/viro.1993.1653].

Transient HTLV-I infection of a human glioma cell line following cell-free exposure

GRAZIANI, GRAZIA;FARAONI, ISABELLA;BONMASSAR, ENZO;MACCHI, BEATRICE
1993-12-01

Abstract

The human T-lymphotropic virus type I (HTLV-I) has been recently associated with cases of tropical spastic paraparesis and human myelopathy. In order to study whether cells of neuroectodermic origin were susceptible to HTLV-I infection, a human glioma cell line T67 was exposed in vitro to HTLV-I by a cell-free method of virus transmission. The presence of HTLV-I proviral DNA was analyzed by polymerase chain reaction 3, 7, and 14 days after infection. The results showed the presence of LTR, pol, and tax sequences within glioma cell line 3 days after the infection. However after 7 and 14 days, detection of HTLV-I sequences remarkably decreased. P19 expression peaked 7 days after infection and decreased in the following week. These data provide evidence that cell-free transmission of HTLV-I results in transient infection of cells of glial origin.
dic-1993
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore BIO/14 - FARMACOLOGIA
English
Con Impact Factor ISI
gag Gene Products, Human Immunodeficiency Virus; Polymerase Chain Reaction; DNA, Viral; Tumor Cells, Cultured; Retroviridae Proteins, Oncogenic; Neuroglia; HTLV-I Antigens; Humans; Human T-lymphotropic virus 1; Monocytes; Gene Products, gag; Proviruses
Graziani, G., Faraoni, I., Zhang, J., Caronti, B., Lauro, G., Bonmassar, E., et al. (1993). Transient HTLV-I infection of a human glioma cell line following cell-free exposure. VIROLOGY, 197(2), 767-769 [10.1006/viro.1993.1653].
Graziani, G; Faraoni, I; Zhang, J; Caronti, B; Lauro, G; Bonmassar, E; Macchi, B
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/68033
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