Junctional transfer of ions in monolayer primary cultures of Sertoli cells and in intact seminiferous tubules from 20 day-old rats has been investigated by using electrophysiological techniques. The electrotonic coupling of both intact tubule and monolayer culture was inhibited by the presence of dibutyryl cyclic AMP (10(-4) M) in the medium. In contrast, 1-oleoyl-2-acetylglycerol (10(-5) M) and 12-O-tetradecanoyl-phorbol-13-acetate (10(-7) M) decreased the junctional resistivity and increased the extent of the coupling in immature tubules. The significance of this modulation of the cell coupling in the seminiferous epithelium is discussed.

Grassi, F., Monaco, L., Fratamico, G., DOLCI IANNINI, S., Iannini, E., Conti, M., et al. (1986). Putative second messengers affect cell coupling in the seminiferous tubules. CELL BIOLOGY INTERNATIONAL REPORTS, 10(8), 631-639.

Putative second messengers affect cell coupling in the seminiferous tubules

DOLCI IANNINI, SUSANNA;
1986-08-01

Abstract

Junctional transfer of ions in monolayer primary cultures of Sertoli cells and in intact seminiferous tubules from 20 day-old rats has been investigated by using electrophysiological techniques. The electrotonic coupling of both intact tubule and monolayer culture was inhibited by the presence of dibutyryl cyclic AMP (10(-4) M) in the medium. In contrast, 1-oleoyl-2-acetylglycerol (10(-5) M) and 12-O-tetradecanoyl-phorbol-13-acetate (10(-7) M) decreased the junctional resistivity and increased the extent of the coupling in immature tubules. The significance of this modulation of the cell coupling in the seminiferous epithelium is discussed.
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore BIO/16
English
Con Impact Factor ISI
Animals; Tetradecanoylphorbol Acetate; Testis; Cyclic AMP; Diglycerides; Rats; Rats, Inbred Strains; Sertoli Cells; Cells, Cultured; Cell Communication; Seminiferous Tubules; Bucladesine; Male
Grassi, F., Monaco, L., Fratamico, G., DOLCI IANNINI, S., Iannini, E., Conti, M., et al. (1986). Putative second messengers affect cell coupling in the seminiferous tubules. CELL BIOLOGY INTERNATIONAL REPORTS, 10(8), 631-639.
Grassi, F; Monaco, L; Fratamico, G; DOLCI IANNINI, S; Iannini, E; Conti, M; Eusebi, F; Stefanini, M
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/67767
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