The immunogenicity and the protection induced by an hepatitis delta virus (HDV) vaccine consisting of the small nucleoprotein (HDAg) (p24) and adjuvanted with MF59 or Freund's adjuvant (FA) were evaluated in woodchucks chronically infected with woodchuck hepatitis virus (WHV) and challenged with hepatitis delta virus. Humoral and T-cell-mediated responses to HDAg were measured. Anti-HD antibodies appeared earlier in the FA/p24 animals. After challenge, all MF59/p24 vaccinated animals showed a response to HDAg-derived peptides, compared to two of the five FA/p24 animals and one of the control animals. Serum HDV-RNA peak values and persistence were considerably reduced in immunized animals, in comparison to controls. Furthermore, HDV-RNA was absent in autopsy liver tissues of 50% of the MF59/p24 animals, whereas high levels were present in all of the FA/p24 animals and controls. Histological liver analysis performed before and after challenge revealed the presence of acute hepatitis-like lesions only in the controls. Overall, the results suggest that the MF59/p24 vaccine better controls the infection in terms of viral replication and survival. © 2003 Elsevier Ltd. All rights reserved.

D'Ugo, E., Paroli, M., Palmieri, G., Giuseppetti, R., Tritarelli, E., Bruni, R., et al. (2004). Immunization of woodchucks with adjuvanted sHDAg (p24): immune response and outcome following challenge. VACCINE, 22, 457-466 [10.1016/j.vaccine.2003.07.001].

Immunization of woodchucks with adjuvanted sHDAg (p24): immune response and outcome following challenge

PALMIERI, GIAMPIERO;
2004

Abstract

The immunogenicity and the protection induced by an hepatitis delta virus (HDV) vaccine consisting of the small nucleoprotein (HDAg) (p24) and adjuvanted with MF59 or Freund's adjuvant (FA) were evaluated in woodchucks chronically infected with woodchuck hepatitis virus (WHV) and challenged with hepatitis delta virus. Humoral and T-cell-mediated responses to HDAg were measured. Anti-HD antibodies appeared earlier in the FA/p24 animals. After challenge, all MF59/p24 vaccinated animals showed a response to HDAg-derived peptides, compared to two of the five FA/p24 animals and one of the control animals. Serum HDV-RNA peak values and persistence were considerably reduced in immunized animals, in comparison to controls. Furthermore, HDV-RNA was absent in autopsy liver tissues of 50% of the MF59/p24 animals, whereas high levels were present in all of the FA/p24 animals and controls. Histological liver analysis performed before and after challenge revealed the presence of acute hepatitis-like lesions only in the controls. Overall, the results suggest that the MF59/p24 vaccine better controls the infection in terms of viral replication and survival. © 2003 Elsevier Ltd. All rights reserved.
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore MED/08 - Anatomia Patologica
English
Con Impact Factor ISI
Adjuvants; Hepatitis delta virus (HDV); Woodchuck hepatitis virus (WHV); Woodchuck immunization
antigen p24; Freund adjuvant; hepatitis delta antigen; hepatitis delta virus vaccine; hepatitis vaccine; immunological adjuvant; nucleoprotein; unclassified drug; animal experiment; animal model; animal tissue; antibody blood level; antibody titer; article; cellular immunity; controlled study; delta agent hepatitis; dose response; drug dose regimen; humoral immunity; immune response; immunization; immunogenicity; liver function test; nonhuman; priority journal; virus replication; virus survival; woodchuck; Adjuvants, Immunologic; Animals; Hepatitis Antigens; Hepatitis B; Hepatitis B Antibodies; Hepatitis B Virus, Woodchuck; Liver; Marmota; Reverse Transcriptase Polymerase Chain Reaction; RNA, Viral; Survival Analysis; T-Lymphocytes; Vaccination; Vaccines, Synthetic
D'Ugo, E., Paroli, M., Palmieri, G., Giuseppetti, R., Tritarelli, E., Bruni, R., et al. (2004). Immunization of woodchucks with adjuvanted sHDAg (p24): immune response and outcome following challenge. VACCINE, 22, 457-466 [10.1016/j.vaccine.2003.07.001].
D'Ugo, E; Paroli, M; Palmieri, G; Giuseppetti, R; Argentini, C; Tritarelli, E; Bruni, R; Barnaba, V; Houghton, M; Rapicetta, M
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2108/67633
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