We have studied in vitro protein synthesis in mouse denuded and follicle-enclosed oocytes by measuring 3H-leucine incorporation into TCA-precipitable material. Thirty percent more radiolabelled TCA-precipitable material was found in follicle-enclosed oocytes. Since in our experimental conditions the internal specific activity of 3H-leucine does not differ in the two systems, and no appreciable transfer of labelled protein from follicle cells to the oocyte occurs, we conclude that follicle cells have a beneficial influence on the oocyte's protein synthetic activity.

Salustri, A., Martinozzi, M. (1983). A comparison of protein synthetic activity in in vitro cultured denuded and follicle-enclosed oocytes. CELL BIOLOGY INTERNATIONAL REPORTS, 7(12), 1049-1055.

A comparison of protein synthetic activity in in vitro cultured denuded and follicle-enclosed oocytes

SALUSTRI, ANTONIETTA;
1983-12-01

Abstract

We have studied in vitro protein synthesis in mouse denuded and follicle-enclosed oocytes by measuring 3H-leucine incorporation into TCA-precipitable material. Thirty percent more radiolabelled TCA-precipitable material was found in follicle-enclosed oocytes. Since in our experimental conditions the internal specific activity of 3H-leucine does not differ in the two systems, and no appreciable transfer of labelled protein from follicle cells to the oocyte occurs, we conclude that follicle cells have a beneficial influence on the oocyte's protein synthetic activity.
dic-1983
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore BIO/17 - ISTOLOGIA
English
Con Impact Factor ISI
Animals; Protein Biosynthesis; Cells, Cultured; Ovarian Follicle; Oocytes; Leucine; Mice; Female
Salustri, A., Martinozzi, M. (1983). A comparison of protein synthetic activity in in vitro cultured denuded and follicle-enclosed oocytes. CELL BIOLOGY INTERNATIONAL REPORTS, 7(12), 1049-1055.
Salustri, A; Martinozzi, M
Articolo su rivista
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/67156
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