We have studied in vitro protein synthesis in mouse denuded and follicle-enclosed oocytes by measuring 3H-leucine incorporation into TCA-precipitable material. Thirty percent more radiolabelled TCA-precipitable material was found in follicle-enclosed oocytes. Since in our experimental conditions the internal specific activity of 3H-leucine does not differ in the two systems, and no appreciable transfer of labelled protein from follicle cells to the oocyte occurs, we conclude that follicle cells have a beneficial influence on the oocyte's protein synthetic activity.
Salustri, A., Martinozzi, M. (1983). A comparison of protein synthetic activity in in vitro cultured denuded and follicle-enclosed oocytes. CELL BIOLOGY INTERNATIONAL REPORTS, 7(12), 1049-1055.
A comparison of protein synthetic activity in in vitro cultured denuded and follicle-enclosed oocytes
SALUSTRI, ANTONIETTA;
1983-12-01
Abstract
We have studied in vitro protein synthesis in mouse denuded and follicle-enclosed oocytes by measuring 3H-leucine incorporation into TCA-precipitable material. Thirty percent more radiolabelled TCA-precipitable material was found in follicle-enclosed oocytes. Since in our experimental conditions the internal specific activity of 3H-leucine does not differ in the two systems, and no appreciable transfer of labelled protein from follicle cells to the oocyte occurs, we conclude that follicle cells have a beneficial influence on the oocyte's protein synthetic activity.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
