During apoptosis, an increase in cytosolic Ca(2+) concentration ([Ca(2+)](c)) accompanies the depletion of endoplasmic reticulum (ER). The actual roles of each of the two events in apoptosis are difficult to understand. In this work, we have modulated the basal [Ca(2+)](c) and the thapsigargin (THG)-dependent reticular flux (i.e., by chelating extracellular Ca(2+) or by modulating intracellular Ca(2+) by 3-aminobenzamide [3-ABA]). We have found that these treatments alter these Ca(2+) parameters in a differential way and, accordingly, affect apoptosis differentially. We have found that the increase in [Ca(2+)](c) is related to the extent of apoptosis, whereas the ER depletion affects the apoptotic nuclear morphology by shifting it towards the cleavage mode.

Cerella, C., D'Alessio, M., DE NICOLA, M., Magrini, A., Bergamaschi, A., Ghibelli, L. (2003). Cytosolic and endoplasmic reticulum Ca2+ concentrations determine the extent and the morphological type of apoptosis, respectively. ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 1010, 74-77.

Cytosolic and endoplasmic reticulum Ca2+ concentrations determine the extent and the morphological type of apoptosis, respectively

DE NICOLA, MILENA;MAGRINI, ANDREA;BERGAMASCHI, ANTONIO;GHIBELLI, LINA
2003-12-01

Abstract

During apoptosis, an increase in cytosolic Ca(2+) concentration ([Ca(2+)](c)) accompanies the depletion of endoplasmic reticulum (ER). The actual roles of each of the two events in apoptosis are difficult to understand. In this work, we have modulated the basal [Ca(2+)](c) and the thapsigargin (THG)-dependent reticular flux (i.e., by chelating extracellular Ca(2+) or by modulating intracellular Ca(2+) by 3-aminobenzamide [3-ABA]). We have found that these treatments alter these Ca(2+) parameters in a differential way and, accordingly, affect apoptosis differentially. We have found that the increase in [Ca(2+)](c) is related to the extent of apoptosis, whereas the ER depletion affects the apoptotic nuclear morphology by shifting it towards the cleavage mode.
dic-2003
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore MED/44 - MEDICINA DEL LAVORO
English
Con Impact Factor ISI
Apoptosis; Calcium; Egtazic Acid; Endoplasmic Reticulum; Humans; Cell Nucleus; Benzamides; Puromycin; Cytosol; Flow Cytometry; Etoposide; Calcium Signaling; U937 Cells
Cerella, C., D'Alessio, M., DE NICOLA, M., Magrini, A., Bergamaschi, A., Ghibelli, L. (2003). Cytosolic and endoplasmic reticulum Ca2+ concentrations determine the extent and the morphological type of apoptosis, respectively. ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 1010, 74-77.
Cerella, C; D'Alessio, M; DE NICOLA, M; Magrini, A; Bergamaschi, A; Ghibelli, L
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/67055
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