Association between extremely low frequency electromagnetic fields (ELF-EMF) and human cancers is controversial, and few studies have been conducted on their influence on oncogenic viruses. We studied the effects of 1 mT, 50 Hz sine waves, applied for 24-72 h, on Kaposi's sarcoma (KS)-associated herpesvirus (KSHV or HHV-8) in BCBL-1, a latently infected primary effusion lymphoma (PEL) cell line. ELF-EMF exposure did not affect the growth and viability of BCBL-1 cells, either stimulated or not with TPA. The total amount of KSHV DNA detected in ELF-EMF exposed cultures not stimulated with TPA did not differ from that of the unexposed controls (P = ns). However, in the presence of TPA stimulation, total KSHV DNA content was found higher in ELF-EMF exposed than in control BCBL-1 cultures (P = .024) at 72 h exposure, but not earlier. Viral DNA increase significantly correlated with increased mean fluorescence intensity/cell for the lytic antigen gp K8.1A/B (P < .01), but not with percentage of gp K8.1A/B-positive cells or of cells containing virions. Viral progeny produced under ELF-EMF exposure consisted mainly of defective viral particles.

Pica, F., Serafino, A., Divizia, M., Donia, D., Fraschetti, M., Sinibaldi-Salimei, P., et al. (2006). Effect of extremely low frequency electromagnetic fields (ELF-EMF) on Kaposi's sarcoma-associated herpes virus in BCBL-1 cells. BIOELECTROMAGNETICS, 27(3), 226-232 [10.1002/bem.20198].

Effect of extremely low frequency electromagnetic fields (ELF-EMF) on Kaposi's sarcoma-associated herpes virus in BCBL-1 cells

PICA, FRANCESCA;DIVIZIA, MAURIZIO;DONIA, DOMENICA TOMMASA;Sinibaldi Salimei, P;GIGANTI, MARIA GABRIELLA;VOLPI, ANTONIO
2006-04

Abstract

Association between extremely low frequency electromagnetic fields (ELF-EMF) and human cancers is controversial, and few studies have been conducted on their influence on oncogenic viruses. We studied the effects of 1 mT, 50 Hz sine waves, applied for 24-72 h, on Kaposi's sarcoma (KS)-associated herpesvirus (KSHV or HHV-8) in BCBL-1, a latently infected primary effusion lymphoma (PEL) cell line. ELF-EMF exposure did not affect the growth and viability of BCBL-1 cells, either stimulated or not with TPA. The total amount of KSHV DNA detected in ELF-EMF exposed cultures not stimulated with TPA did not differ from that of the unexposed controls (P = ns). However, in the presence of TPA stimulation, total KSHV DNA content was found higher in ELF-EMF exposed than in control BCBL-1 cultures (P = .024) at 72 h exposure, but not earlier. Viral DNA increase significantly correlated with increased mean fluorescence intensity/cell for the lytic antigen gp K8.1A/B (P < .01), but not with percentage of gp K8.1A/B-positive cells or of cells containing virions. Viral progeny produced under ELF-EMF exposure consisted mainly of defective viral particles.
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore MED/42 - Igiene Generale e Applicata
English
Virus Replication; Herpesvirus 8, Human; Radiation Dosage; DNA, Viral; Electromagnetic Fields; Humans; Cell Line, Tumor; Electricity; Dose-Response Relationship, Radiation; Lymphoma; Cell Survival
Pica, F., Serafino, A., Divizia, M., Donia, D., Fraschetti, M., Sinibaldi-Salimei, P., et al. (2006). Effect of extremely low frequency electromagnetic fields (ELF-EMF) on Kaposi's sarcoma-associated herpes virus in BCBL-1 cells. BIOELECTROMAGNETICS, 27(3), 226-232 [10.1002/bem.20198].
Pica, F; Serafino, A; Divizia, M; Donia, Dt; Fraschetti, M; Sinibaldi Salimei, P; Giganti, Mg; Volpi, A
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2108/66929
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