Liver peroxisomes during prenatal development were studied by means of morphological and morphometric analysis in foetuses growing both in untreated and in clofibrate-treated rats. Pregnant rats were fed a standard diet (25 g/d) containing or not 0.8% clofibrate during the week preceding sacrifice and livers were excised from 13, 15, 17, 19 and 21-day old foetuses and newborn rats. The morphometric analysis of hepatocyte peroxisomes, performed by a semiautomatic method in specimens incubated with 3,3' diaminobenzidine, shows that the peroxisome volume density and average diameter in test animals were significantly increased over the control values. While the increase in the average diameter was roughly the same (X 1.4) at all examined stages, the volume density increased over the control values particularly in foetuses over 19d-old and in newborn rats; over the same period the peroxisome numerical density also significantly increased over the control values. Finally, the average diameters of peroxisome profiles in test rats showed a more dispersed distribution (SD 40%) than in control animals (SD 30%). These results demonstrate that clofibrate, given to rats during pregnancy, induces peroxisomal proliferation in the livers of their foetuses. Data are discussed in view of the models proposed for peroxisomal biogenesis.

Stefanini, S., Mauriello, A., Farrace, M.g., Cibelli, A., Ceru, M. (1989). Proliferative response of foetal liver peroxisomes to clofibrate treatment of pregnant rats. A quantitative evaluation. BIOLOGY OF THE CELL, 67(3), 299-305.

Proliferative response of foetal liver peroxisomes to clofibrate treatment of pregnant rats. A quantitative evaluation

MAURIELLO, ALESSANDRO;FARRACE, MARIA GRAZIA;
1989-01-01

Abstract

Liver peroxisomes during prenatal development were studied by means of morphological and morphometric analysis in foetuses growing both in untreated and in clofibrate-treated rats. Pregnant rats were fed a standard diet (25 g/d) containing or not 0.8% clofibrate during the week preceding sacrifice and livers were excised from 13, 15, 17, 19 and 21-day old foetuses and newborn rats. The morphometric analysis of hepatocyte peroxisomes, performed by a semiautomatic method in specimens incubated with 3,3' diaminobenzidine, shows that the peroxisome volume density and average diameter in test animals were significantly increased over the control values. While the increase in the average diameter was roughly the same (X 1.4) at all examined stages, the volume density increased over the control values particularly in foetuses over 19d-old and in newborn rats; over the same period the peroxisome numerical density also significantly increased over the control values. Finally, the average diameters of peroxisome profiles in test rats showed a more dispersed distribution (SD 40%) than in control animals (SD 30%). These results demonstrate that clofibrate, given to rats during pregnancy, induces peroxisomal proliferation in the livers of their foetuses. Data are discussed in view of the models proposed for peroxisomal biogenesis.
1989
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore MED/08 - Anatomia Patologica
English
Con Impact Factor ISI
Rats, Inbred Strains; Rats; Clofibrate; Fetus; Animals; Liver; Microscopy, Electron; Microbodies; Cell Division
Stefanini, S., Mauriello, A., Farrace, M.g., Cibelli, A., Ceru, M. (1989). Proliferative response of foetal liver peroxisomes to clofibrate treatment of pregnant rats. A quantitative evaluation. BIOLOGY OF THE CELL, 67(3), 299-305.
Stefanini, S; Mauriello, A; Farrace, Mg; Cibelli, A; Ceru, M
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/66798
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