A factor produced by the oocytes induces in vitro FSH-treated cumulus and mural granulosa cells to synthesize hyaluronic acid. In the present study we examined hyaluronic acid synthesis by the two cell populations in vivo. After injection of hCG into PMSG-primed immature mice, cumulus and mural granulosa cells adjacent to the antrum synthesized a large amount of such glycosaminoglycan, while the outermost layers layers of mural granulosa cells did not. The results suggest that in vivo differences in hyaluronic acid synthesis between follicle cell subpopulations depend on a diffusion gradient of the oocyte factor. We also identified a proteoglycan species synthesized by cumulus cells which may contribute to hyaluronic acid organization in the intercellular spaces.

Salustri, A., Camaioni, A., Tirone, E., D'Alessandris, C. (1995). Hyaluronic acid and proteoglycan accumulation in the cumulus oophorus matrix. ITALIAN JOURNAL OF ANATOMY AND EMBRYOLOGY, 100 Suppl 1, 479-484.

Hyaluronic acid and proteoglycan accumulation in the cumulus oophorus matrix

SALUSTRI, ANTONIETTA;CAMAIONI, ANTONELLA;
1995-01-01

Abstract

A factor produced by the oocytes induces in vitro FSH-treated cumulus and mural granulosa cells to synthesize hyaluronic acid. In the present study we examined hyaluronic acid synthesis by the two cell populations in vivo. After injection of hCG into PMSG-primed immature mice, cumulus and mural granulosa cells adjacent to the antrum synthesized a large amount of such glycosaminoglycan, while the outermost layers layers of mural granulosa cells did not. The results suggest that in vivo differences in hyaluronic acid synthesis between follicle cell subpopulations depend on a diffusion gradient of the oocyte factor. We also identified a proteoglycan species synthesized by cumulus cells which may contribute to hyaluronic acid organization in the intercellular spaces.
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore BIO/17
English
Proteoglycans; Granulosa Cells; Animals; Ovulation; Estrus; Hyaluronic Acid; Cells, Cultured; Extracellular Matrix; Mice; Chorionic Gonadotropin; Female
Salustri, A., Camaioni, A., Tirone, E., D'Alessandris, C. (1995). Hyaluronic acid and proteoglycan accumulation in the cumulus oophorus matrix. ITALIAN JOURNAL OF ANATOMY AND EMBRYOLOGY, 100 Suppl 1, 479-484.
Salustri, A; Camaioni, A; Tirone, E; D'Alessandris, C
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/66792
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