Several human and animal cell lines have been used to grow hepatitis E virus. The strain SAR-55 was adapted only on PLF/PLC/5 cell line without any visible cytopathic effect. The growth of the SAR-55 was monitored by examining the positive and the negative strands of HEV-RNA. Stool samples, obtained from hospitalised acute hepatitis patients at the Fever Hospital of Alexandria (Egypt), were used to confirm the susceptibility of PLF/PLC/5 cells. After more than one-week's cultivation, three stool samples out of 17 IgM anti-HEV positive and 1 from 52 IgG anti-HEV positive patients showed a specific RT-PCR amplification product. The nucleotide sequences of the methyltransferase region of the genome in the isolates revealed the maximum homology with Burma strain with several point mutations.

Divizia, M., Gabrieli, R., Degener, A., Renganathan, E., Pillot, J., Stefanoni, M., et al. (1999). Evidence of hepatitis E virus replication on cell cultures. NEW MICROBIOLOGICA, 22(2), 77-83.

Evidence of hepatitis E virus replication on cell cultures

DIVIZIA, MAURIZIO;GABRIELI, ROSANNA;PANA', AUGUSTO
1999-04-01

Abstract

Several human and animal cell lines have been used to grow hepatitis E virus. The strain SAR-55 was adapted only on PLF/PLC/5 cell line without any visible cytopathic effect. The growth of the SAR-55 was monitored by examining the positive and the negative strands of HEV-RNA. Stool samples, obtained from hospitalised acute hepatitis patients at the Fever Hospital of Alexandria (Egypt), were used to confirm the susceptibility of PLF/PLC/5 cells. After more than one-week's cultivation, three stool samples out of 17 IgM anti-HEV positive and 1 from 52 IgG anti-HEV positive patients showed a specific RT-PCR amplification product. The nucleotide sequences of the methyltransferase region of the genome in the isolates revealed the maximum homology with Burma strain with several point mutations.
apr-1999
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore MED/42 - IGIENE GENERALE E APPLICATA
English
Virus Replication; Animals; Hepatitis E; Humans; Hepatitis E virus; Sequence Analysis, DNA; Reverse Transcriptase Polymerase Chain Reaction; RNA, Viral; Virus Cultivation; DNA, Complementary; Base Sequence; Sequence Alignment; Molecular Sequence Data; Feces; Methyltransferases; Cell Line
Divizia, M., Gabrieli, R., Degener, A., Renganathan, E., Pillot, J., Stefanoni, M., et al. (1999). Evidence of hepatitis E virus replication on cell cultures. NEW MICROBIOLOGICA, 22(2), 77-83.
Divizia, M; Gabrieli, R; Degener, A; Renganathan, E; Pillot, J; Stefanoni, M; el Ghazzawi, E; Kader, O; Gamil, F; el Sawaf, G; Saleh, E; el Sherbini, ...espandi
Articolo su rivista
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/66734
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 9
  • ???jsp.display-item.citation.isi??? 6
social impact