Two strains of hepatitis A virus (HAV) were isolated in cell culture and found to induce a cytopathic effect at early passages. The nucleotide sequences of the 5' non-translated region (5'NTR) and of genes 2B, 2C, 3A and 3B were determined for these strains and found to contain mutations similar to those detected in cell-culture adapted variants of HAV strain HM175. In addition, gene 3A shows a deletion of three aspartic acid residues near the N-terminus of the polypeptide. In combination with variations in the 5'NTR and in genes 2B and 2C, the absence of an aspartic acid residue in position 4 of gene 3A of three cytopathic clones of HM175 suggests a possible role of the 3A protein in determining the cytopathic phenotype.

Morace, G., Pisani, G., Beneduce, F., Divizia, M., Pana', A. (1993). Mutations in the 3A genomic region of two cytopathic strains of hepatitis A virus isolated in Italy. VIRUS RESEARCH, 28(2), 187-194.

Mutations in the 3A genomic region of two cytopathic strains of hepatitis A virus isolated in Italy

DIVIZIA, MAURIZIO;PANA', AUGUSTO
1993-05-01

Abstract

Two strains of hepatitis A virus (HAV) were isolated in cell culture and found to induce a cytopathic effect at early passages. The nucleotide sequences of the 5' non-translated region (5'NTR) and of genes 2B, 2C, 3A and 3B were determined for these strains and found to contain mutations similar to those detected in cell-culture adapted variants of HAV strain HM175. In addition, gene 3A shows a deletion of three aspartic acid residues near the N-terminus of the polypeptide. In combination with variations in the 5'NTR and in genes 2B and 2C, the absence of an aspartic acid residue in position 4 of gene 3A of three cytopathic clones of HM175 suggests a possible role of the 3A protein in determining the cytopathic phenotype.
mag-1993
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore MED/42 - IGIENE GENERALE E APPLICATA
English
Base Sequence; Humans; Molecular Sequence Data; Hepatovirus; Genes, Viral; Cytopathogenic Effect, Viral; Aspartic Acid; Mutation; Italy; Gene Deletion
Morace, G., Pisani, G., Beneduce, F., Divizia, M., Pana', A. (1993). Mutations in the 3A genomic region of two cytopathic strains of hepatitis A virus isolated in Italy. VIRUS RESEARCH, 28(2), 187-194.
Morace, G; Pisani, G; Beneduce, F; Divizia, M; Pana', A
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/66723
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