Tumor promonocytic U937 cells cultured under a low O(2)/high CO(2) atmosphere display altered characteristics after restoration of normal atmosphere: increased resistance to apoptosis induced by different treatments; apoptotic morphology; lack of glutathione (GSH) extrusion in apoptosis; lack of protection by antioxidants; and lack of Ca(2+) mobilization with thapsigargin. These alterations were stably maintained for many months of culture in normal conditions, originating the stable U937-HX variant. Since the hypoxic treatment did not produce a great selective pressure, the alterations are conceivably the result of stable adaptative response.
DE NICOLA, M., Liuzzi, F., Cerella, C., D'Alessio, M., Bergamaschi, A., Magrini, A., et al. (2003). Hypoxic stress stably alters apoptotic parameters on U937 cells. ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 1010, 426-429.
Hypoxic stress stably alters apoptotic parameters on U937 cells
DE NICOLA, MILENA;BERGAMASCHI, ANTONIO;MAGRINI, ANDREA;GHIBELLI, LINA
2003-12-01
Abstract
Tumor promonocytic U937 cells cultured under a low O(2)/high CO(2) atmosphere display altered characteristics after restoration of normal atmosphere: increased resistance to apoptosis induced by different treatments; apoptotic morphology; lack of glutathione (GSH) extrusion in apoptosis; lack of protection by antioxidants; and lack of Ca(2+) mobilization with thapsigargin. These alterations were stably maintained for many months of culture in normal conditions, originating the stable U937-HX variant. Since the hypoxic treatment did not produce a great selective pressure, the alterations are conceivably the result of stable adaptative response.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
