The effect of some cellular function inhibitors on hepatitis A virus (HAV) adsorption and on the successive events of infection in a monkey cell line (Frp/3 cells) was investigated. Treatments of Frp/3 cells with colcemide, vinblastine and cytochalasin D, which affect cytoskeleton organization, indicated that neither microtubules nor microfilaments play an important role in the early events of HAV infection. Monensin, which acts as an ionophore on intracellular vesicle compartments inhibited HAV infection probably at the uncoating step. Inhibition of viral replication to a different degree was observed with both inhibitors of oxidative phosphorylation, such as dinitrophenol and sodium azide, and with an inhibitor of glycolysis, 2-deoxy-D-glucose. However, none of these compounds significantly affected the early steps of infection, thus demonstrating that HAV replication is largely dependent upon cell energy.

Superti, F., Seganti, L., Orsi, N., Divizia, M., Gabrieli, R., Pana', A. (1989). Effect of cellular function inhibitors on the infection of Frp/3 cells by hepatitis A virus. MEDICAL MICROBIOLOGY AND IMMUNOLOGY, 178(1), 29-36.

Effect of cellular function inhibitors on the infection of Frp/3 cells by hepatitis A virus

DIVIZIA, MAURIZIO;GABRIELI, ROSANNA;PANA', AUGUSTO
1989-01-01

Abstract

The effect of some cellular function inhibitors on hepatitis A virus (HAV) adsorption and on the successive events of infection in a monkey cell line (Frp/3 cells) was investigated. Treatments of Frp/3 cells with colcemide, vinblastine and cytochalasin D, which affect cytoskeleton organization, indicated that neither microtubules nor microfilaments play an important role in the early events of HAV infection. Monensin, which acts as an ionophore on intracellular vesicle compartments inhibited HAV infection probably at the uncoating step. Inhibition of viral replication to a different degree was observed with both inhibitors of oxidative phosphorylation, such as dinitrophenol and sodium azide, and with an inhibitor of glycolysis, 2-deoxy-D-glucose. However, none of these compounds significantly affected the early steps of infection, thus demonstrating that HAV replication is largely dependent upon cell energy.
1989
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore MED/42 - IGIENE GENERALE E APPLICATA
English
Virus Replication; Cytoskeleton; Azides; Dose-Response Relationship, Drug; Cells, Cultured; Hydrogen-Ion Concentration; Humans; Dinitrophenols; Hepatovirus; Sodium Azide; Monensin; Cytochalasin B
Superti, F., Seganti, L., Orsi, N., Divizia, M., Gabrieli, R., Pana', A. (1989). Effect of cellular function inhibitors on the infection of Frp/3 cells by hepatitis A virus. MEDICAL MICROBIOLOGY AND IMMUNOLOGY, 178(1), 29-36.
Superti, F; Seganti, L; Orsi, N; Divizia, M; Gabrieli, R; Pana', A
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/66654
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