It was long believed that melatonin might counteract intracellular oxidative stress because it was shown to potentiate antioxidant endogenous defences, and to increase the activity of many antioxidant enzymes. However, it is now becoming evident that when radicals are measured within cells, melatonin increases, rather than decreasing, radical production. Herein we demonstrate a pro-oxidant effect of melatonin in U937 cells by showing an increase of intracellular oxidative species and a depletion of glutathione (GSH). The activity of glutathione peroxidase is not modified by melatonin treatment as it does occur in other experimental models.

Albertini, M., Radogna, F., Accorsi, A., Uguccioni, F., Paternoster, L., Cerella, C., et al. (2006). Intracellular pro-oxidant activity of melatonin deprives U937 cells of reduced glutathione without affecting glutathione peroxidase activity. ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 1091, 10-16 [10.1196/annals.1378.050].

Intracellular pro-oxidant activity of melatonin deprives U937 cells of reduced glutathione without affecting glutathione peroxidase activity

RADOGNA, FLAVIA;DE NICOLA, MILENA;BERGAMASCHI, ANTONIO;MAGRINI, ANDREA;GHIBELLI, LINA
2006-12-01

Abstract

It was long believed that melatonin might counteract intracellular oxidative stress because it was shown to potentiate antioxidant endogenous defences, and to increase the activity of many antioxidant enzymes. However, it is now becoming evident that when radicals are measured within cells, melatonin increases, rather than decreasing, radical production. Herein we demonstrate a pro-oxidant effect of melatonin in U937 cells by showing an increase of intracellular oxidative species and a depletion of glutathione (GSH). The activity of glutathione peroxidase is not modified by melatonin treatment as it does occur in other experimental models.
dic-2006
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore MED/44 - MEDICINA DEL LAVORO
English
Con Impact Factor ISI
Oxidation-Reduction; Intracellular Fluid; Glutathione; Enzyme Activation; Humans; Glutathione Peroxidase; Melatonin; Reactive Oxygen Species; Oxidants; U937 Cells
Albertini, M., Radogna, F., Accorsi, A., Uguccioni, F., Paternoster, L., Cerella, C., et al. (2006). Intracellular pro-oxidant activity of melatonin deprives U937 cells of reduced glutathione without affecting glutathione peroxidase activity. ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 1091, 10-16 [10.1196/annals.1378.050].
Albertini, M; Radogna, F; Accorsi, A; Uguccioni, F; Paternoster, L; Cerella, C; DE NICOLA, M; D'Alessio, M; Bergamaschi, A; Magrini, A; Ghibelli, L
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/66625
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