The lethality caused by calcium-free medium (CFM) to fully grown mouse oocytes significantly decreases if a disulfide reducing agent (dithiothreitol, reduced glutathione, or L-cysteine) is added to the medium. In this condition, most of the surviving oocytes do not spontaneously resume meiosis. We also show that the sulfhydryl content of fully grown oocytes, estimated by monobromobimane labeling, rapidly decreases during culture in CFM. The hypothesis is discussed that lethality of oocytes cultured in CFM may be a consequence of an alteration of thiol-disulfide balance.

DE FELICI, M., DOLCI IANNINI, S., Siracusa, G. (1987). Involvement of thiol-disulfide groups in the sensitivity of fully grown mouse oocytes to calcium-free medium. JOURNAL OF EXPERIMENTAL ZOOLOGY, 243(2), 283-287 [10.1002/jez.1402430213].

Involvement of thiol-disulfide groups in the sensitivity of fully grown mouse oocytes to calcium-free medium

DE FELICI, MASSIMO;DOLCI IANNINI, SUSANNA;SIRACUSA, GREGORIO
1987-08-01

Abstract

The lethality caused by calcium-free medium (CFM) to fully grown mouse oocytes significantly decreases if a disulfide reducing agent (dithiothreitol, reduced glutathione, or L-cysteine) is added to the medium. In this condition, most of the surviving oocytes do not spontaneously resume meiosis. We also show that the sulfhydryl content of fully grown oocytes, estimated by monobromobimane labeling, rapidly decreases during culture in CFM. The hypothesis is discussed that lethality of oocytes cultured in CFM may be a consequence of an alteration of thiol-disulfide balance.
ago-1987
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore BIO/17 - ISTOLOGIA
English
Con Impact Factor ISI
Meiosis; Cells, Cultured; Female; Mice; Sulfhydryl Compounds; Disulfides; Oocytes; Animals; Calcium
DE FELICI, M., DOLCI IANNINI, S., Siracusa, G. (1987). Involvement of thiol-disulfide groups in the sensitivity of fully grown mouse oocytes to calcium-free medium. JOURNAL OF EXPERIMENTAL ZOOLOGY, 243(2), 283-287 [10.1002/jez.1402430213].
DE FELICI, M; DOLCI IANNINI, S; Siracusa, G
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/66244
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