The synthesis and intracellular distribution of actin were studied in isolated dictyate and metaphase II mouse oocytes by 1) sodium dodecyl sulfate-polyacrylamide gel electrophoresis of newly synthetized oocyte protein and 2) cytochemical F-actin labeling by fluorescent phalloidin. Unpermeabilized, fully grown oocytes bound phalloidin intensely at the level of the zona pellucida (ZP), such ZP-associated actin representing a significant portion of total actin found in these cells. In contrast, phalloidin binding to ZP was very low in growing oocytes and was undetectable in ovulated, metaphase II eggs. When ZP-associated actin of fully grown oocytes was removed by prolongedly exposing oocytes to alpha-chymotrypsin, the amount of newly synthesized actin displayed by cumulus-enclosed oocytes was reduced to a level comparable to that shown by oocytes isolated from granulosa cells. We demonstrate that ZP-associated actin belongs to granulosa cell processes that remain within the ZP as a consequence of oocyte isolation procedures. We conclude that actin synthesis of mouse oocytes is not regulated by granulosa cells.

Canipari, R., Bevilacqua, A., Colonna, R., DE FELICI, M., Mangia, F. (1988). Actin synthesis is not regulated by granulosa cells in mouse growing and preovulatory oocytes. GAMETE RESEARCH, 20(2), 115-124 [10.1002/mrd.1120200202].

Actin synthesis is not regulated by granulosa cells in mouse growing and preovulatory oocytes

DE FELICI, MASSIMO;
1988

Abstract

The synthesis and intracellular distribution of actin were studied in isolated dictyate and metaphase II mouse oocytes by 1) sodium dodecyl sulfate-polyacrylamide gel electrophoresis of newly synthetized oocyte protein and 2) cytochemical F-actin labeling by fluorescent phalloidin. Unpermeabilized, fully grown oocytes bound phalloidin intensely at the level of the zona pellucida (ZP), such ZP-associated actin representing a significant portion of total actin found in these cells. In contrast, phalloidin binding to ZP was very low in growing oocytes and was undetectable in ovulated, metaphase II eggs. When ZP-associated actin of fully grown oocytes was removed by prolongedly exposing oocytes to alpha-chymotrypsin, the amount of newly synthesized actin displayed by cumulus-enclosed oocytes was reduced to a level comparable to that shown by oocytes isolated from granulosa cells. We demonstrate that ZP-associated actin belongs to granulosa cell processes that remain within the ZP as a consequence of oocyte isolation procedures. We conclude that actin synthesis of mouse oocytes is not regulated by granulosa cells.
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore BIO/17
English
Con Impact Factor ISI
Actins; Zona Pellucida; Follicular Phase; Phalloidine; Female; Mice; Granulosa Cells; Oligopeptides; Oocytes; Animals; Ovum
Canipari, R., Bevilacqua, A., Colonna, R., DE FELICI, M., Mangia, F. (1988). Actin synthesis is not regulated by granulosa cells in mouse growing and preovulatory oocytes. GAMETE RESEARCH, 20(2), 115-124 [10.1002/mrd.1120200202].
Canipari, R; Bevilacqua, A; Colonna, R; DE FELICI, M; Mangia, F
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/66243
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