In the present paper we investigate whether the adhesion that occurs in vitro between mouse fetal germ cells and follicle cells is accompanied by the establishment of cell coupling between the two cell types. The possible coupling between germ cells that spontaneously aggregate in culture has also been monitored. When germ cells, isolated from the gonads of 12.5-13.5-day-post coitum embryos, were loaded with 6-carboxyfluorescein diacetate and seeded onto follicle cell monolayers dye was transferred from the germ cells to the adjacent somatic cells within 2-3 h of culture. No dye transfer was observed when germ cells were seeded on fibroblast monolayers or between members of homotypic germ cell aggregates. These results indicate that, following cell to cell adhesion, fetal germ cells are able to establish heterocellular gap junctions with somatic cells of gonadal origin in culture.

DE FELICI, M., DOLCI IANNINI, S., Siracusa, G. (1989). Fetal germ cells establish cell coupling with follicle cells in vitro. CELL DIFFERENTIATION AND DEVELOPMENT, 28(1), 65-69.

Fetal germ cells establish cell coupling with follicle cells in vitro

DE FELICI, MASSIMO;DOLCI IANNINI, SUSANNA;SIRACUSA, GREGORIO
1989-10-01

Abstract

In the present paper we investigate whether the adhesion that occurs in vitro between mouse fetal germ cells and follicle cells is accompanied by the establishment of cell coupling between the two cell types. The possible coupling between germ cells that spontaneously aggregate in culture has also been monitored. When germ cells, isolated from the gonads of 12.5-13.5-day-post coitum embryos, were loaded with 6-carboxyfluorescein diacetate and seeded onto follicle cell monolayers dye was transferred from the germ cells to the adjacent somatic cells within 2-3 h of culture. No dye transfer was observed when germ cells were seeded on fibroblast monolayers or between members of homotypic germ cell aggregates. These results indicate that, following cell to cell adhesion, fetal germ cells are able to establish heterocellular gap junctions with somatic cells of gonadal origin in culture.
ott-1989
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore BIO/17 - ISTOLOGIA
English
Con Impact Factor ISI
Intercellular Junctions; Male; Cell Communication; Testis; Female; Mice; Mice, Inbred Strains; Cell Adhesion; Ovary; Ovarian Follicle; Animals
DE FELICI, M., DOLCI IANNINI, S., Siracusa, G. (1989). Fetal germ cells establish cell coupling with follicle cells in vitro. CELL DIFFERENTIATION AND DEVELOPMENT, 28(1), 65-69.
DE FELICI, M; DOLCI IANNINI, S; Siracusa, G
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/66238
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