The synthesis of protein-bound carbohydrates has been studied in primordial germ cells (PGCs) and in somatic cells of 12.5 to 13.5-days-postcoitum (dpc) fetal mouse gonads. Both cell types were shown to synthesize asparagine-linked glycopeptides and glycosaminoglycans (GAGs). In addition, PGCs also synthesize lactosaminoglycans (LAGs) although in different proportions in female and male germ cells. Female PGCs, which at 13.5 dpc are entering meiosis, synthesize mainly LAGs, and minor amounts of hyaluronic acid (HA) and chondroitin sulfate (CS). Male germ cells, on the other hand, synthesize mainly CS. Furthermore, somatic cells of fetal gonads synthesize HA as the major class of GAGs. It is suggested that the activation of LAG synthesis in developing germ cells might be related to the beginning of meiosis. Moreover, we propose that HA synthesis might be developmentally regulated in somatic cells of the gonad, in order to regulate the establishment of specific interactions with germ cells.

DE FELICI, M., Boitani, C., Cossu, G. (1985). Synthesis of glycoconjugates in mouse primordial germ cells. DEVELOPMENTAL BIOLOGY, 109(2), 375-380.

Synthesis of glycoconjugates in mouse primordial germ cells

DE FELICI, MASSIMO;
1985-06-01

Abstract

The synthesis of protein-bound carbohydrates has been studied in primordial germ cells (PGCs) and in somatic cells of 12.5 to 13.5-days-postcoitum (dpc) fetal mouse gonads. Both cell types were shown to synthesize asparagine-linked glycopeptides and glycosaminoglycans (GAGs). In addition, PGCs also synthesize lactosaminoglycans (LAGs) although in different proportions in female and male germ cells. Female PGCs, which at 13.5 dpc are entering meiosis, synthesize mainly LAGs, and minor amounts of hyaluronic acid (HA) and chondroitin sulfate (CS). Male germ cells, on the other hand, synthesize mainly CS. Furthermore, somatic cells of fetal gonads synthesize HA as the major class of GAGs. It is suggested that the activation of LAG synthesis in developing germ cells might be related to the beginning of meiosis. Moreover, we propose that HA synthesis might be developmentally regulated in somatic cells of the gonad, in order to regulate the establishment of specific interactions with germ cells.
giu-1985
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore BIO/17 - ISTOLOGIA
English
Con Impact Factor ISI
Germ Cells; Male; Glycopeptides; Spermatogonia; Female; Animals; Glycosaminoglycans; Amino Sugars; Organ Culture Techniques; Gonads; Hyaluronic Acid; Mice; Polysaccharides
DE FELICI, M., Boitani, C., Cossu, G. (1985). Synthesis of glycoconjugates in mouse primordial germ cells. DEVELOPMENTAL BIOLOGY, 109(2), 375-380.
DE FELICI, M; Boitani, C; Cossu, G
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/66230
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