Adhesiveness of mouse primordial germ cells to follicular and Sertoli cell monolayers

The adhesiveness of female and male mouse primordial germ cells (PGCs) to somatic cell monolayers of various origin has been studied in the definite conditions of an in vitro system. PGCs were isolated from the gonads of embryos of various post coital ages according to the method of De Felici & McLaren (1982), and seeded on the cell monolayers. PGCs from 12.5 to 15.5 days post coitum (dpc) embryos specifically adhered to Sertoli and follicular cells obtained from adult gonads. The percentage of female PGCs which adhered to follicular cell monolayers was significantly higher than that of male PGCs. No significant adhesion was seen between PGCs and somatic cell monolayers obtained from various embryonic and adult tissues. The results obtained indicate that the simple in vitro assay described in the present paper might help to characterize the cellular interactions between somatic and germ cells during gonadal development.