We show by RT-PCR analysis that transcripts from the Xist gene, which is normally expressed from the inactive X chromosome of female somatic cells and postnatal male germ cells, are transiently expressed also in male embryos around the time of testis differentiation, mainly in somatic cells of urogenital ridges. In the postnatal testis, we find that Xist transcripts are mainly localized within the nucleus of haploid spermatids. These findings suggest that inactivation of the X-chromosome might occur not only in adult male germ cells, but also, transiently, in somatic cells of the male urogenital ridge. Both in the embryonal gonad and in differentiating germ cells Xist expression in males overlaps the pattern of expression of the testis determining gene Sry.

DOLCI IANNINI, S., Geremia, R., Albanesi, C., Rossi, P. (1994). Expression of the Xist gene in urogenital ridges of midgestation male embryos. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 205(1), 334-340 [10.1006/bbrc.1994.2669].

Expression of the Xist gene in urogenital ridges of midgestation male embryos

DOLCI IANNINI, SUSANNA;GEREMIA, RAFFAELE;ROSSI, PELLEGRINO
1994-11-30

Abstract

We show by RT-PCR analysis that transcripts from the Xist gene, which is normally expressed from the inactive X chromosome of female somatic cells and postnatal male germ cells, are transiently expressed also in male embryos around the time of testis differentiation, mainly in somatic cells of urogenital ridges. In the postnatal testis, we find that Xist transcripts are mainly localized within the nucleus of haploid spermatids. These findings suggest that inactivation of the X-chromosome might occur not only in adult male germ cells, but also, transiently, in somatic cells of the male urogenital ridge. Both in the embryonal gonad and in differentiating germ cells Xist expression in males overlaps the pattern of expression of the testis determining gene Sry.
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore BIO/16
English
Con Impact Factor ISI
Animals; Oligodeoxyribonucleotides; Gestational Age; Mice; RNA, Untranslated; RNA, Messenger; Base Sequence; X Chromosome; Transcription Factors; Urogenital System; Molecular Sequence Data; Embryo, Mammalian; Gene Expression Regulation, Developmental; Male
DOLCI IANNINI, S., Geremia, R., Albanesi, C., Rossi, P. (1994). Expression of the Xist gene in urogenital ridges of midgestation male embryos. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 205(1), 334-340 [10.1006/bbrc.1994.2669].
DOLCI IANNINI, S; Geremia, R; Albanesi, C; Rossi, P
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/65817
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