Reverse transcriptase polymerase chain reaction (RT-PCR) analysis shows that Sry mRNA is expressed in male fetal urogenital ridges from 12.5 day p.c. embryos, but not in enriched populations of primordial germ cells from the same embryos, indicating that Sry is expressed in the somatic cells of the embryonal gonad at the time of testis determination. We also show that, in the adult male mouse testis, Sry mRNA is expressed at high levels in meiotic and postmeiotic germ cells and, at much lower levels, also in Sertoli cells. Treatment with cyclic adenosine monophosphate (cAMP) analogs of cultured Sertoli cells from postnatal testis completely abolishes Sry mRNA expression.

Rossi, P., DOLCI IANNINI, S., Albanesi, C., Grimaldi, P., Geremia, R. (1993). Direct evidence that the mouse sex-determining gene Sry is expressed in the somatic cells of male fetal gonads and in the germ cell line in the adult testis. MOLECULAR REPRODUCTION AND DEVELOPMENT, 34(4), 369-373 [10.1002/mrd.1080340404].

Direct evidence that the mouse sex-determining gene Sry is expressed in the somatic cells of male fetal gonads and in the germ cell line in the adult testis

ROSSI, PELLEGRINO;DOLCI IANNINI, SUSANNA;GRIMALDI, PAOLA;GEREMIA, RAFFAELE
1993-04-01

Abstract

Reverse transcriptase polymerase chain reaction (RT-PCR) analysis shows that Sry mRNA is expressed in male fetal urogenital ridges from 12.5 day p.c. embryos, but not in enriched populations of primordial germ cells from the same embryos, indicating that Sry is expressed in the somatic cells of the embryonal gonad at the time of testis determination. We also show that, in the adult male mouse testis, Sry mRNA is expressed at high levels in meiotic and postmeiotic germ cells and, at much lower levels, also in Sertoli cells. Treatment with cyclic adenosine monophosphate (cAMP) analogs of cultured Sertoli cells from postnatal testis completely abolishes Sry mRNA expression.
apr-1993
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore BIO/16 - ANATOMIA UMANA
English
Con Impact Factor ISI
Animals; Testis; Proto-Oncogene Proteins c-kit; Spermatozoa; Gene Expression; Mice; RNA, Messenger; Hypoxanthine Phosphoribosyltransferase; DNA Probes; Polymerase Chain Reaction; Base Sequence; Sertoli Cells; Proto-Oncogene Proteins; DNA; Molecular Sequence Data; Sex Determination Analysis; Bucladesine; Female; Male
Rossi, P., DOLCI IANNINI, S., Albanesi, C., Grimaldi, P., Geremia, R. (1993). Direct evidence that the mouse sex-determining gene Sry is expressed in the somatic cells of male fetal gonads and in the germ cell line in the adult testis. MOLECULAR REPRODUCTION AND DEVELOPMENT, 34(4), 369-373 [10.1002/mrd.1080340404].
Rossi, P; DOLCI IANNINI, S; Albanesi, C; Grimaldi, P; Geremia, R
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/65812
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