The role of DNA polymerase alpha-DNA primase complex and DNA polymerase beta in DNA replication and ultraviolet-induced DNA repair synthesis has been analyzed in mouse spermatogenesis. Autoradiographic experiments with germ cells in culture, indicating an involvement of DNA polymerase alpha and/or delta in DNA replication, and of DNA polymerase beta in DNA repair synthesis, have been confirmed by studying partially purified enzymes. These findings support the idea that, different from other biological systems, in meiotic and post meiotic male mouse germ cells DNA polymerase beta is the main DNA polymerase form needed for DNA repair.

Orlando, P., Geremia, R., Frusciante, C., Tedeschi, B., Grippo, P. (1988). DNA repair synthesis in mouse spermatogenesis involves DNA polymerase beta activity. CELL DIFFERENTIATION, 23(3), 221-230 [10.1016/0045-6039(88)90075-9].

DNA repair synthesis in mouse spermatogenesis involves DNA polymerase beta activity

GEREMIA, RAFFAELE;TEDESCHI, BRUNA;
1988-04-01

Abstract

The role of DNA polymerase alpha-DNA primase complex and DNA polymerase beta in DNA replication and ultraviolet-induced DNA repair synthesis has been analyzed in mouse spermatogenesis. Autoradiographic experiments with germ cells in culture, indicating an involvement of DNA polymerase alpha and/or delta in DNA replication, and of DNA polymerase beta in DNA repair synthesis, have been confirmed by studying partially purified enzymes. These findings support the idea that, different from other biological systems, in meiotic and post meiotic male mouse germ cells DNA polymerase beta is the main DNA polymerase form needed for DNA repair.
apr-1988
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore BIO/16 - ANATOMIA UMANA
English
Con Impact Factor ISI
Animals; Ultraviolet Rays; Thymine Nucleotides; Cell Extracts; DNA Polymerase I; DNA Repair; Electrophoresis, Polyacrylamide Gel; Cell Differentiation; Mice; Autoradiography; Spermatogenesis; DNA Primase; Mice, Inbred Strains; Ethylmaleimide; Aphidicolin; Diterpenes; Chromatography, DEAE-Cellulose; RNA Nucleotidyltransferases; DNA Polymerase II; Male; DNA Replication
Orlando, P., Geremia, R., Frusciante, C., Tedeschi, B., Grippo, P. (1988). DNA repair synthesis in mouse spermatogenesis involves DNA polymerase beta activity. CELL DIFFERENTIATION, 23(3), 221-230 [10.1016/0045-6039(88)90075-9].
Orlando, P; Geremia, R; Frusciante, C; Tedeschi, B; Grippo, P
Articolo su rivista
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/65788
Citazioni
  • ???jsp.display-item.citation.pmc??? 4
  • Scopus 11
  • ???jsp.display-item.citation.isi??? ND
social impact