Isolated male germ cells of the mouse possess a heat-stable stimulatory activity of Ca2+-dependent, calmodulin-free phosphodiesterase. Ionic exchange chromatography allowed partial purification of the activator and the isolation of multiple forms of phosphodiesterase stimulation inhibitor. The activator has been identified as calmodulin on the basis of chromatographic behaviour and electrophoretic mobility. Quantitative analysis showed variations of calmodulin levels at different stages of spermatogenesis. Quantitative analysis of cyclic nucleotide hydrolysis in germ cell cytosol showed that the activity of Ca2+-dependent phosphodiesterase is different in meiotic and post-meiotic mouse male germ cells. These data suggest that calcium-dependent pathway and a Ca2+-dependent regulation of cyclic nucleotides are present in developing germ cells.

D'Agostino, A., Monaco, L., Conti, M., Geremia, R. (1983). Calmodulin in mouse male germ cells: a qualitative and quantitative study. CELL DIFFERENTIATION, 13(1), 35-40.

Calmodulin in mouse male germ cells: a qualitative and quantitative study

GEREMIA, RAFFAELE
1983-09-01

Abstract

Isolated male germ cells of the mouse possess a heat-stable stimulatory activity of Ca2+-dependent, calmodulin-free phosphodiesterase. Ionic exchange chromatography allowed partial purification of the activator and the isolation of multiple forms of phosphodiesterase stimulation inhibitor. The activator has been identified as calmodulin on the basis of chromatographic behaviour and electrophoretic mobility. Quantitative analysis showed variations of calmodulin levels at different stages of spermatogenesis. Quantitative analysis of cyclic nucleotide hydrolysis in germ cell cytosol showed that the activity of Ca2+-dependent phosphodiesterase is different in meiotic and post-meiotic mouse male germ cells. These data suggest that calcium-dependent pathway and a Ca2+-dependent regulation of cyclic nucleotides are present in developing germ cells.
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore BIO/16
English
Con Impact Factor ISI
Animals; Phosphoric Diester Hydrolases; Spermatids; Spermatozoa; Calmodulin; Mice; Spermatocytes; Male
D'Agostino, A., Monaco, L., Conti, M., Geremia, R. (1983). Calmodulin in mouse male germ cells: a qualitative and quantitative study. CELL DIFFERENTIATION, 13(1), 35-40.
D'Agostino, A; Monaco, L; Conti, M; Geremia, R
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/65739
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