Although a wealth of evidence supports the hypothesis that some functions of the nervous system may be altered during exposure to microgravity, the possible changes in basic neuronal physiology are not easy to assess. Indeed, few studies have examined whether microgravity affects the development of neurons in culture. In the present study, a suspension of dissociated cortical cells from rat embryos were exposed to 24 h of simulated microgravity before plating in a normal adherent culture system. Both preexposed and control cells were used after a period of 7-10 d in vitro. The vitality and the level of reactive oxygen species of cultures previously exposed did not differ from those of normal cultures. Cellular characterization by immunostaining with a specific antibody displayed normal neuronal phenotype in control cells, whereas pretreatment in simulated microgravity revealed an increase of glial fibrillary acidic protein fluorescence in the elongated stellate glial cells. Electrophysiological recording indicated that the electrical properties of neurons preexposed were comparable with those of controls. Overall, our results indicate that a short time of simulated microgravity preexposure does not affect dramatically the ability of dissociated neural cells to develop and differentiate in an adherent culture system.

Crestini, A., Zona, C., Sebastiani, P., Pieri, M., Caracciolo, V., Malvezzi Campeggi, L., et al. (2004). Effects of simulated microgravity on the development and maturation of dissociated cortical neurons. IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY. ANIMAL, 40(5-6), 159-165 [10.1290/1543-706X(2004)40<159:EOSMOT>2.0.CO;2].

Effects of simulated microgravity on the development and maturation of dissociated cortical neurons

ZONA, CRISTINA;PIERI, MASSIMO;
2004-01-01

Abstract

Although a wealth of evidence supports the hypothesis that some functions of the nervous system may be altered during exposure to microgravity, the possible changes in basic neuronal physiology are not easy to assess. Indeed, few studies have examined whether microgravity affects the development of neurons in culture. In the present study, a suspension of dissociated cortical cells from rat embryos were exposed to 24 h of simulated microgravity before plating in a normal adherent culture system. Both preexposed and control cells were used after a period of 7-10 d in vitro. The vitality and the level of reactive oxygen species of cultures previously exposed did not differ from those of normal cultures. Cellular characterization by immunostaining with a specific antibody displayed normal neuronal phenotype in control cells, whereas pretreatment in simulated microgravity revealed an increase of glial fibrillary acidic protein fluorescence in the elongated stellate glial cells. Electrophysiological recording indicated that the electrical properties of neurons preexposed were comparable with those of controls. Overall, our results indicate that a short time of simulated microgravity preexposure does not affect dramatically the ability of dissociated neural cells to develop and differentiate in an adherent culture system.
2004
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore BIO/09 - FISIOLOGIA
English
Con Impact Factor ISI
Animals; Microtubule-Associated Proteins; Cell Differentiation; Glial Fibrillary Acidic Protein; Electrophysiology; Reactive Oxygen Species; Cell Survival; Pregnancy; Rats; Cerebral Cortex; Neurons; Weightlessness Simulation; Embryo, Mammalian; Immunohistochemistry; Female
Crestini, A., Zona, C., Sebastiani, P., Pieri, M., Caracciolo, V., Malvezzi Campeggi, L., et al. (2004). Effects of simulated microgravity on the development and maturation of dissociated cortical neurons. IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY. ANIMAL, 40(5-6), 159-165 [10.1290/1543-706X(2004)40<159:EOSMOT>2.0.CO;2].
Crestini, A; Zona, C; Sebastiani, P; Pieri, M; Caracciolo, V; Malvezzi Campeggi, L; Confaloni, A; Di Loreto, S
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/65507
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