Whole-cell, patch-clamp recordings were used to study voltage-gated currents generated by cerebellar granule cells that were cultured in medium containing either 10% fetal calf serum (hereafter termed S + granules) or neurite outgrowth and adhesion complex (NOAC, hereafter called NOAC granules). NOAC is a protein complex found in rabbit serum that renders granules resistant to the excitotoxic action of excitatory amino acids. During depolarizing commands both S+ and NOAC granules generated Na+ and Ca2+ inward currents and an early and a late K+ outward currents. However, Na+ and Ca2+ inward currents and late outward K+ currents recorded in NOAC granules were smaller than those seen in S+ granules. Furthermore, although of similar amplitude, early K+ currents displayed different kinetics in the two types of neurons. Thus, these data demonstrate that the electrophysiological properties of cerebellar granules, and probably of other neuronal populations, depend upon serum components and raise the possibility that an analogous modulation might be operative in vivo, and play a role in development, synaptic plasticity or neuropathological processes.

Zona, C., Ragozzino, D., Ciotti, M., Mercanti, D., Avoli, M., Brancati, A., et al. (1993). Sodium, calcium and late potassium currents are reduced in cerebellar granule cells cultured in the presence of a protein complex conferring resistance to excitatory amino acids. EUROPEAN JOURNAL OF NEUROSCIENCE, 5(11), 1479-1484 [10.1111/j.1460-9568.1993.tb00215.x].

Sodium, calcium and late potassium currents are reduced in cerebellar granule cells cultured in the presence of a protein complex conferring resistance to excitatory amino acids

ZONA, CRISTINA;BRANCATI, ALDO;CALISSANO, PIETRO
1993-11-01

Abstract

Whole-cell, patch-clamp recordings were used to study voltage-gated currents generated by cerebellar granule cells that were cultured in medium containing either 10% fetal calf serum (hereafter termed S + granules) or neurite outgrowth and adhesion complex (NOAC, hereafter called NOAC granules). NOAC is a protein complex found in rabbit serum that renders granules resistant to the excitotoxic action of excitatory amino acids. During depolarizing commands both S+ and NOAC granules generated Na+ and Ca2+ inward currents and an early and a late K+ outward currents. However, Na+ and Ca2+ inward currents and late outward K+ currents recorded in NOAC granules were smaller than those seen in S+ granules. Furthermore, although of similar amplitude, early K+ currents displayed different kinetics in the two types of neurons. Thus, these data demonstrate that the electrophysiological properties of cerebellar granules, and probably of other neuronal populations, depend upon serum components and raise the possibility that an analogous modulation might be operative in vivo, and play a role in development, synaptic plasticity or neuropathological processes.
1-nov-1993
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore BIO/09 - FISIOLOGIA
English
Con Impact Factor ISI
Animals; Cerebellum; Drug Resistance; Tetrodotoxin; Nerve Growth Factors; Potassium Channels; Sodium Channels; Calcium Channels; Rats; Cells, Cultured; Kinetics; Neurons; Rats, Wistar; Tetraethylammonium Compounds; Membrane Potentials; 4-Aminopyridine; Tetraethylammonium; Neurotoxins
Zona, C., Ragozzino, D., Ciotti, M., Mercanti, D., Avoli, M., Brancati, A., et al. (1993). Sodium, calcium and late potassium currents are reduced in cerebellar granule cells cultured in the presence of a protein complex conferring resistance to excitatory amino acids. EUROPEAN JOURNAL OF NEUROSCIENCE, 5(11), 1479-1484 [10.1111/j.1460-9568.1993.tb00215.x].
Zona, C; Ragozzino, D; Ciotti, M; Mercanti, D; Avoli, M; Brancati, A; Calissano, P
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/65211
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