Hydrolysis of Leu-enkephalin and association to cells of the radioactive label carried by this peptide have been studied on the K562(S) erythroleukaemic cell subline. Data obtained indicate that in the presence of these cells Leu-enkephalin is rapidly hydrolyzed and that the peptide's radioactive label is partially associated to the cells. Both phenomena are characterized by distinctly diphasic kinetics, and are oppositely modified by cells' stimulated erythroid differentiation. In the presence of proteolysis inhibitors, hydrolysis of Leu-enkephalin and association to cells of its radioactive label are reduced. These data, indicating that enkephalin hydrolysis is a prerequisite for the association of the radiolabel to cells, are consistent with those obtained with other cell lines of different origins and with human mononucleate cells. The modifications induced by differentiation of cells on hydrolysis, association and membrane conductance can be interpreted as an indication of extensive modifications of the membrane structures responsible for the mechanisms controlling those phenomena.

Urbani, A., Bongiorno, L., Marini, M.a., Zona, C., Roda, L. (1994). Hydrolysis of leucine enkephalin and its association to the K562(S) erythroleukaemic cell line. IMMUNOPHARMACOLOGY, 27(2), 119-130 [10.1016/0162-3109(94)90046-9].

Hydrolysis of leucine enkephalin and its association to the K562(S) erythroleukaemic cell line

URBANI, ALESSANDRA;BONGIORNO, LAVINIA;MARINI, MARIA ADELAIDE;ZONA, CRISTINA;
1994-01-01

Abstract

Hydrolysis of Leu-enkephalin and association to cells of the radioactive label carried by this peptide have been studied on the K562(S) erythroleukaemic cell subline. Data obtained indicate that in the presence of these cells Leu-enkephalin is rapidly hydrolyzed and that the peptide's radioactive label is partially associated to the cells. Both phenomena are characterized by distinctly diphasic kinetics, and are oppositely modified by cells' stimulated erythroid differentiation. In the presence of proteolysis inhibitors, hydrolysis of Leu-enkephalin and association to cells of its radioactive label are reduced. These data, indicating that enkephalin hydrolysis is a prerequisite for the association of the radiolabel to cells, are consistent with those obtained with other cell lines of different origins and with human mononucleate cells. The modifications induced by differentiation of cells on hydrolysis, association and membrane conductance can be interpreted as an indication of extensive modifications of the membrane structures responsible for the mechanisms controlling those phenomena.
1994
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore BIO/09 - FISIOLOGIA
English
Con Impact Factor ISI
Leukemia, Erythroblastic, Acute; Tumor Cells, Cultured; Humans; Erythrocytes; Molecular Sequence Data; Membrane Potentials; Cell Differentiation; Enkephalin, Leucine; Amino Acid Sequence; Hydrolysis; Protease Inhibitors
Urbani, A., Bongiorno, L., Marini, M.a., Zona, C., Roda, L. (1994). Hydrolysis of leucine enkephalin and its association to the K562(S) erythroleukaemic cell line. IMMUNOPHARMACOLOGY, 27(2), 119-130 [10.1016/0162-3109(94)90046-9].
Urbani, A; Bongiorno, L; Marini, Ma; Zona, C; Roda, L
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/65210
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