23-Nor-deoxycholic acid is widely used as internal standard in gas-liquid chromatographic studies of bile acids. Two batches of this compound, submitted to conventional alkaline hydrolysis of bile acid conjugates, were found to be transformed into a product with chromatographic properties different from those of "authentic" 23-nor-deoxycholic acid. To identify this "new" product a comparison was made between chromatographic properties, mass spectra, and NMR spectra of 23-nor-deoxycholic acid before and after alkaline hydrolysis. The results indicate that the "new" compound is the true 23-nor-deoxycholic acid while the product present in the two batches examined is a monoacetate derivative.
Attili, A., Angelico, M., Capocaccia, L., Pièche, U., Cantafora, A. (1982). Drawbacks in the use of 23-nor-deoxycholic acid standards. JOURNAL OF LIPID RESEARCH, 23(1), 211-215.
Drawbacks in the use of 23-nor-deoxycholic acid standards
ANGELICO, MARIO;
1982-01-01
Abstract
23-Nor-deoxycholic acid is widely used as internal standard in gas-liquid chromatographic studies of bile acids. Two batches of this compound, submitted to conventional alkaline hydrolysis of bile acid conjugates, were found to be transformed into a product with chromatographic properties different from those of "authentic" 23-nor-deoxycholic acid. To identify this "new" product a comparison was made between chromatographic properties, mass spectra, and NMR spectra of 23-nor-deoxycholic acid before and after alkaline hydrolysis. The results indicate that the "new" compound is the true 23-nor-deoxycholic acid while the product present in the two batches examined is a monoacetate derivative.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
