Mono or binocular deprivation, during early postnatal development, produces dramatic effects on the anatomy and physiology of the visual system. Here we report that dark exposure induces apoptotic cell death in the lateral geniculate nucleus (LGN) of adult rabbit and this may be related to activation of N-methyl-D-aspartate (NMDA) and non-NMDA subtypes of glutamate receptors. In situ DNA fragmentation (TUNEL) was observed in the LGN of rabbits exposed to dark for 48 h. Morphological changes were confirmed on hematoxylin-eosin stained brain tissue coronal sections. Systemic treatment with CGP 040116 or MK 801, two NMDA receptor antagonists, and with GYKI 52466, a non-NMDA receptor antagonist, prevented in situ DNA fragmentation and nuclear chromatin marginalization and condensation. In no instance was apoptosis seen in rabbits kept under a normal light-dark cycle. Our findings indicate that glutamate, acting on NMDA and non-NMDA receptors, may be involved in the mechanisms of apoptotic cell death induced in the LGN of adult rabbits exposed to darkness.

Nucci, C., Piccirilli, S., Bagetta, G., Nisticò, G., Cerulli, L. (1997). N-methyl-D-aspartate (NMDA) and non-NMDA glutamate receptor antagonists protect from apoptosis induced in the lateral geniculate nucleus of rabbits exposed to the dark. NEUROSCIENCE LETTERS, 229(3), 185-188.

N-methyl-D-aspartate (NMDA) and non-NMDA glutamate receptor antagonists protect from apoptosis induced in the lateral geniculate nucleus of rabbits exposed to the dark

NUCCI, CARLO;PICCIRILLI, SILVIA;CERULLI, LUCIANO
1997-07-04

Abstract

Mono or binocular deprivation, during early postnatal development, produces dramatic effects on the anatomy and physiology of the visual system. Here we report that dark exposure induces apoptotic cell death in the lateral geniculate nucleus (LGN) of adult rabbit and this may be related to activation of N-methyl-D-aspartate (NMDA) and non-NMDA subtypes of glutamate receptors. In situ DNA fragmentation (TUNEL) was observed in the LGN of rabbits exposed to dark for 48 h. Morphological changes were confirmed on hematoxylin-eosin stained brain tissue coronal sections. Systemic treatment with CGP 040116 or MK 801, two NMDA receptor antagonists, and with GYKI 52466, a non-NMDA receptor antagonist, prevented in situ DNA fragmentation and nuclear chromatin marginalization and condensation. In no instance was apoptosis seen in rabbits kept under a normal light-dark cycle. Our findings indicate that glutamate, acting on NMDA and non-NMDA receptors, may be involved in the mechanisms of apoptotic cell death induced in the LGN of adult rabbits exposed to darkness.
4-lug-1997
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore MED/30 - MALATTIE APPARATO VISIVO
English
Darkness; Animals; Apoptosis; Rabbits; Geniculate Bodies; Benzodiazepines; Dizocilpine Maleate; Deoxyuracil Nucleotides; Vision, Monocular; Anti-Anxiety Agents; Staining and Labeling; Receptors, N-Methyl-D-Aspartate; Biotin; DNA Fragmentation; Male; Excitatory Amino Acid Antagonists
http://dx.doi.org/10.1016/S0304-3940(97)00455-2,
Nucci, C., Piccirilli, S., Bagetta, G., Nisticò, G., Cerulli, L. (1997). N-methyl-D-aspartate (NMDA) and non-NMDA glutamate receptor antagonists protect from apoptosis induced in the lateral geniculate nucleus of rabbits exposed to the dark. NEUROSCIENCE LETTERS, 229(3), 185-188.
Nucci, C; Piccirilli, S; Bagetta, G; Nisticò, G; Cerulli, L
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/64789
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