Fusicoccin (FC) bindingsites solubilised from microsomal fractions of spinach leaves have been entrapped into soybean lecithin liposomes with an 80% yield. The investigation of the properties of these proteoliposomes has demonstrated that the rates of FC-binding and of exchange between radioactive and cold FC are intermediate between those observed with membrane-bound and with solubilised bindingsites. It appears that the entrapped proteins are preferentially outside-oriented since they are inactivated by trypsin treatment; moreover, Triton X-100 permeabilization of the proteoliposomes demonstrated that one fifth of these sites are inside-oriented.
Aducci, P., Ballio, A., Fullone, M., & Persichetti, F. (1986). Entrapment into liposomes of fusicoccin binding sites. PLANT SCIENCE, 45, 83-86.
Tipologia: | Articolo su rivista |
Citazione: | Aducci, P., Ballio, A., Fullone, M., & Persichetti, F. (1986). Entrapment into liposomes of fusicoccin binding sites. PLANT SCIENCE, 45, 83-86. |
Lingua: | English |
Settore Scientifico Disciplinare: | Settore BIO/04 |
Revisione (peer review): | Esperti anonimi |
Tipo: | Articolo |
Rilevanza: | Rilevanza internazionale |
Digital Object Identifier (DOI): | http://dx.doi.org/10.1016/0168-9452(86)90041-5 |
Stato di pubblicazione: | Pubblicato |
Data di pubblicazione: | 1986 |
Titolo: | Entrapment into liposomes of fusicoccin binding sites |
Autori: | |
Autori: | Aducci, P; Ballio, A; Fullone, M; Persichetti, F |
Appare nelle tipologie: | 01 - Articolo su rivista |