Fusicoccin (FC) bindingsites solubilised from microsomal fractions of spinach leaves have been entrapped into soybean lecithin liposomes with an 80% yield. The investigation of the properties of these proteoliposomes has demonstrated that the rates of FC-binding and of exchange between radioactive and cold FC are intermediate between those observed with membrane-bound and with solubilised bindingsites. It appears that the entrapped proteins are preferentially outside-oriented since they are inactivated by trypsin treatment; moreover, Triton X-100 permeabilization of the proteoliposomes demonstrated that one fifth of these sites are inside-oriented.

Aducci, P., Ballio, A., Fullone, M., Persichetti, F. (1986). Entrapment into liposomes of fusicoccin binding sites. PLANT SCIENCE, 45, 83-86 [10.1016/0168-9452(86)90041-5].

Entrapment into liposomes of fusicoccin binding sites

ADUCCI, PATRIZIA;
1986-01-01

Abstract

Fusicoccin (FC) bindingsites solubilised from microsomal fractions of spinach leaves have been entrapped into soybean lecithin liposomes with an 80% yield. The investigation of the properties of these proteoliposomes has demonstrated that the rates of FC-binding and of exchange between radioactive and cold FC are intermediate between those observed with membrane-bound and with solubilised bindingsites. It appears that the entrapped proteins are preferentially outside-oriented since they are inactivated by trypsin treatment; moreover, Triton X-100 permeabilization of the proteoliposomes demonstrated that one fifth of these sites are inside-oriented.
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore BIO/04
English
fusicoccin, fusicoccin receptor, liposomes
Aducci, P., Ballio, A., Fullone, M., Persichetti, F. (1986). Entrapment into liposomes of fusicoccin binding sites. PLANT SCIENCE, 45, 83-86 [10.1016/0168-9452(86)90041-5].
Aducci, P; Ballio, A; Fullone, M; Persichetti, F
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/64447
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