A reinvestigatio of fusicoccin (FC) binding properties in plasmalemma-enriched membranes of maizecoleoptiles has been carried out. Scatchard plots of experimental binding data have been analysed by a non-linear least-squares fitting program and are consistent with two classes of independent binding sites. The Kd-values obtained are Kd = 3.3 ± 1.6 × 10−10 M, Kd2 = 1.3 ± 0.53 × 10−8 M and are independent of protein concentration. They are only in partial agreement with those previously obtained by a different method of analysis.

Aducci, P., Coletta, M., Marra, M. (1984). An improved Scatchard analysis of fusicoccin binding to maize coleoptile membranes. PLANT SCIENCE LETTERS, 33, 187-193 [10.1016/0304-4211(84)90008-7].

An improved Scatchard analysis of fusicoccin binding to maize coleoptile membranes.

ADUCCI, PATRIZIA;COLETTA, MASSIMILIANO;MARRA, MAURO
1984

Abstract

A reinvestigatio of fusicoccin (FC) binding properties in plasmalemma-enriched membranes of maizecoleoptiles has been carried out. Scatchard plots of experimental binding data have been analysed by a non-linear least-squares fitting program and are consistent with two classes of independent binding sites. The Kd-values obtained are Kd = 3.3 ± 1.6 × 10−10 M, Kd2 = 1.3 ± 0.53 × 10−8 M and are independent of protein concentration. They are only in partial agreement with those previously obtained by a different method of analysis.
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore BIO/04
Settore BIO/10
English
Aducci, P., Coletta, M., Marra, M. (1984). An improved Scatchard analysis of fusicoccin binding to maize coleoptile membranes. PLANT SCIENCE LETTERS, 33, 187-193 [10.1016/0304-4211(84)90008-7].
Aducci, P; Coletta, M; Marra, M
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/64291
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