A reinvestigatio of fusicoccin (FC) binding properties in plasmalemma-enriched membranes of maizecoleoptiles has been carried out. Scatchard plots of experimental binding data have been analysed by a non-linear least-squares fitting program and are consistent with two classes of independent binding sites. The Kd-values obtained are Kd = 3.3 ± 1.6 × 10−10 M, Kd2 = 1.3 ± 0.53 × 10−8 M and are independent of protein concentration. They are only in partial agreement with those previously obtained by a different method of analysis.
Aducci, P., Coletta, M., Marra, M. (1984). An improved Scatchard analysis of fusicoccin binding to maize coleoptile membranes. PLANT SCIENCE LETTERS, 33, 187-193 [10.1016/0304-4211(84)90008-7].
An improved Scatchard analysis of fusicoccin binding to maize coleoptile membranes.
ADUCCI, PATRIZIA;COLETTA, MASSIMILIANO;MARRA, MAURO
1984-01-01
Abstract
A reinvestigatio of fusicoccin (FC) binding properties in plasmalemma-enriched membranes of maizecoleoptiles has been carried out. Scatchard plots of experimental binding data have been analysed by a non-linear least-squares fitting program and are consistent with two classes of independent binding sites. The Kd-values obtained are Kd = 3.3 ± 1.6 × 10−10 M, Kd2 = 1.3 ± 0.53 × 10−8 M and are independent of protein concentration. They are only in partial agreement with those previously obtained by a different method of analysis.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
