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EnglishBackground-CD40 ligand (CD40L) expression on platelets is mediated by agonists, but the underlying mechanism is still unclear. Methods and Results-CD40L expression was measured in platelets from healthy subjects both with and without the addition of antioxidants or a phospholipase A2 (PLA2) inhibitor and in platelets from 2 patients with an inherited deficiency of gp91phox. Immunoprecipitation analysis was also performed to determine whether normal platelets showed gp91phox expression. Unlike catalase and mannitol, superoxide dismutase inhibited agonist-induced platelet CD40L expression in healthy subjects. Immunoprecipitation analysis also showed that platelets from healthy subjects expressed gp91phox. In 2 male patients with inherited gp91phox deficiency, collagen-, thrombin-, and arachidonic acid-stimulated platelets showed an almost complete absence of superoxide anion (O2-) and CD40L expression. Incubation of platelets from healthy subjects with a PLA2 inhibitor almost completely prevented agonist-induced O2- and CD40L expression. Conclusions-These data provide the first evidence that platelet CD40L expression occurs via arachidonic acid-mediated gp91phox activation.
Pignatelli P., S.V. (2004). gp91phox-dependent expression of platelet CD40 ligand, 110(10), 1326-1329.
| Tipologia: | Articolo su rivista |
| Citazione: | Pignatelli P., S.V. (2004). gp91phox-dependent expression of platelet CD40 ligand, 110(10), 1326-1329. |
| Lingua: | English |
| Settore Scientifico Disciplinare: | Settore MED/09 - Medicina Interna |
| Revisione (peer review): | Sì, ma tipo non specificato |
| Tipo: | Articolo |
| Rilevanza: | Rilevanza internazionale |
| Digital Object Identifier (DOI): | http://dx.doi.org/10.1161/01.CIR.0000134963.77201.55 |
| Stato di pubblicazione: | Pubblicato |
| Data di pubblicazione: | 2004 |
| Titolo: | gp91phox-dependent expression of platelet CD40 ligand |
| Autori: | |
| Autori: | Pignatelli P., Sanguigni V., Lenti L., Ferro D., Finocchi A., Rossi P., Violi F. |
| Appare nelle tipologie: | 01 - Articolo su rivista |
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| File | Descrizione | Tipologia | Licenza | |
|---|---|---|---|---|
| Circulation GP91.pdf | Articolo principale | N/A | Open Access Visualizza/Apri |
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