Giardia duodenalis genotypes and Cryptosporidium species were studied in humans and free-ranging animals living in closed enclaves in Côte d’Ivoire. Three hundred and seven stool samples were tested from humans, and 47 from freely roaming domestic animals (dogs, goats, ducks, chickens). Molecular characterization of the isolates was performed by sequence analysis of a portion of the SSU-rDNA for Giardia and the COWP gene for Cryptosporidium, and a ˇ-giardin SYBR-green real-time PCR was also used to confirm the assignment of Giardia isolates to Assemblages. In humans, genotyping of Giardia assigned many of the sequences (43/56 by the SSU-rDNA gene, and 36/61 by the ˇ-giardin gene) to Assemblage B. The animal species harboured only zoonotic ssemblages A and B, except for dogs, in which host specific Assemblages C and D were also detected. Cryptosporidium meleagridis, C. parvum and C. hominis were detected in humans, while among the animals only chickens were found positive for oocysts, identified as C. meleagridis and C. parvum. The results provide further evidence about the role of freeranging domestic animals living closely with humans in the environmental dissemination and potential transmission of these anthropozoonotic pathogens to humans.

Berrilli, F., D'Alfonso, R., Giangaspero, A., Marangi, M., Brandonisio, O., Kaboré, Y., et al. (2012). Giardia duodenalis genotypes and Cryptosporidium species in humans and domestic animals in Côte d'Ivoire: occurrence and evidence for environmental contamination. TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE, 106(3), 191-195 [10.1016/j.trstmh.2011.12.005].

Giardia duodenalis genotypes and Cryptosporidium species in humans and domestic animals in Côte d'Ivoire: occurrence and evidence for environmental contamination

BERRILLI, FEDERICA;D'ALFONSO, ROSSELLA;LAURO, RENATO;DI CAVE, DAVID
2012

Abstract

Giardia duodenalis genotypes and Cryptosporidium species were studied in humans and free-ranging animals living in closed enclaves in Côte d’Ivoire. Three hundred and seven stool samples were tested from humans, and 47 from freely roaming domestic animals (dogs, goats, ducks, chickens). Molecular characterization of the isolates was performed by sequence analysis of a portion of the SSU-rDNA for Giardia and the COWP gene for Cryptosporidium, and a ˇ-giardin SYBR-green real-time PCR was also used to confirm the assignment of Giardia isolates to Assemblages. In humans, genotyping of Giardia assigned many of the sequences (43/56 by the SSU-rDNA gene, and 36/61 by the ˇ-giardin gene) to Assemblage B. The animal species harboured only zoonotic ssemblages A and B, except for dogs, in which host specific Assemblages C and D were also detected. Cryptosporidium meleagridis, C. parvum and C. hominis were detected in humans, while among the animals only chickens were found positive for oocysts, identified as C. meleagridis and C. parvum. The results provide further evidence about the role of freeranging domestic animals living closely with humans in the environmental dissemination and potential transmission of these anthropozoonotic pathogens to humans.
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore VET/06 - Parassitologia e Malattie Parassitarie degli Animali
Settore BIO/12
English
Con Impact Factor ISI
Giardia duodenalis; cryptosporidium spp.; molecular characterization; environmental contamination; zoonosis; Côte d’Ivoire
Berrilli, F., D'Alfonso, R., Giangaspero, A., Marangi, M., Brandonisio, O., Kaboré, Y., et al. (2012). Giardia duodenalis genotypes and Cryptosporidium species in humans and domestic animals in Côte d'Ivoire: occurrence and evidence for environmental contamination. TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE, 106(3), 191-195 [10.1016/j.trstmh.2011.12.005].
Berrilli, F; D'Alfonso, R; Giangaspero, A; Marangi, M; Brandonisio, O; Kaboré, Y; Glé, C; Cianfanelli, C; Lauro, R; DI CAVE, D
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/58118
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