The aim of this study was to determine whether taurocholate prevents vagotomy-induced cholangiocyte apoptosis. After bile duct ligation (BDL) + vagotomy, rats were fed taurocholate for 1 wk in the absence or presence of wortmannin. Caspase involvement was evaluated by measurement of caspase 8, 9, and 3 activities. Proliferation was determined by morphometry and PCNA immunoblots. Changes in phosphatidylinositol 3-kinase (PI3-kinase) activity were estimated by the expression of the phosphorylated Akt protein. Apically located Na+-dependent bile acid transporter (ABAT) expression and activity were evaluated by immunoblots and [H-3] taurocholate uptake, respectively. Cholangiocyte apoptosis increased, whereas proliferation decreased in BDL + vagotomy rats. Taurocholate feeding prevented vagotomy effects on cholangiocyte functions, which were abolished by wortmannin. ABAT expression and activity as well as phosphorylated Akt protein expression were reduced by vagotomy but restored by taurocholate. The activities of caspase 8, 9, and 3 increased in BDL + vagotomy rats but were restored by taurocholate. The protective effect of taurocholate was associated with maintenance of ABAT activity, downregulation of caspase 8, 9, and 3, and activation of PI3-kinase. Bile acids are important in modulating cholangiocyte proliferation in denervated livers.

Marzioni, M., LeSage, G.D., Glaser, S., Patel, T., Marienfeld, C., Ueno, Y., et al. (2003). Taurocholate prevents the loss of intrahepatic bile ducts due to vagotomy in bile duct-ligated rats. AMERICAN JOURNAL OF PHYSIOLOGY: GASTROINTESTINAL AND LIVER PHYSIOLOGY.

Taurocholate prevents the loss of intrahepatic bile ducts due to vagotomy in bile duct-ligated rats

BAIOCCHI, LEONARDO;
2003

Abstract

The aim of this study was to determine whether taurocholate prevents vagotomy-induced cholangiocyte apoptosis. After bile duct ligation (BDL) + vagotomy, rats were fed taurocholate for 1 wk in the absence or presence of wortmannin. Caspase involvement was evaluated by measurement of caspase 8, 9, and 3 activities. Proliferation was determined by morphometry and PCNA immunoblots. Changes in phosphatidylinositol 3-kinase (PI3-kinase) activity were estimated by the expression of the phosphorylated Akt protein. Apically located Na+-dependent bile acid transporter (ABAT) expression and activity were evaluated by immunoblots and [H-3] taurocholate uptake, respectively. Cholangiocyte apoptosis increased, whereas proliferation decreased in BDL + vagotomy rats. Taurocholate feeding prevented vagotomy effects on cholangiocyte functions, which were abolished by wortmannin. ABAT expression and activity as well as phosphorylated Akt protein expression were reduced by vagotomy but restored by taurocholate. The activities of caspase 8, 9, and 3 increased in BDL + vagotomy rats but were restored by taurocholate. The protective effect of taurocholate was associated with maintenance of ABAT activity, downregulation of caspase 8, 9, and 3, and activation of PI3-kinase. Bile acids are important in modulating cholangiocyte proliferation in denervated livers.
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore MED/12 - Gastroenterologia
English
Con Impact Factor ISI
apoptosis; bile flow; intrahepatic biliary epithelium; proliferation; secretin
ACID-INDUCED APOPTOSIS; CHOLANGIOCYTE GROWTH; LIVER-TRANSPLANTATION; RODENT HEPATOCYTES; EPITHELIAL-CELLS; ACTIVATION; SECRETION; PATHWAY; TRANSPORTER; EXPRESSION
Marzioni, M., LeSage, G.D., Glaser, S., Patel, T., Marienfeld, C., Ueno, Y., et al. (2003). Taurocholate prevents the loss of intrahepatic bile ducts due to vagotomy in bile duct-ligated rats. AMERICAN JOURNAL OF PHYSIOLOGY: GASTROINTESTINAL AND LIVER PHYSIOLOGY.
Marzioni, M; Lesage, Gd; Glaser, S; Patel, T; Marienfeld, C; Ueno, Y; Francis, H; Alvaro, D; Tadlock, L; Benedetti, A; Marucci, L; Baiocchi, L; Phinizy, Jl; Alpini, G
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2108/57936
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