Atopic and non-atopic eczema is an inflammatory cutaneous disease which is common in childhood and is associated with a dysregulation of the immune system. Many genes encoding immune receptors, cytokines, chemokines, chemokine receptors, and adhesion molecules involved in the development of the disease are under the control of transcription factors belonging to the nuclear factor (NF)-kappa B family. To investigate the role of NF-kappa B in the development of eczema, 20 children, affected by relapsing chronic eczema, were enrolled in this study. Eleven of the 20 children showed IgE immunoreactivity and had a positive prick test. The DNA binding activity of NF-kappa B in nuclear extracts of the patients' peripheral blood mononuclear cells (PBMC) was examined by electrophoretic mobility shift assay. We found that basal NF-kappa B-DNA binding activity in PBMC was significantly higher in the eczema patient group in comparison with the same parameter in the healthy age-matched control group. Moreover, we observed a significant correlation between NF-kappa B-DNA binding activity and patients' clinical score (SCORAD). Based on these observations we speculate that NF-kappa B can play an important role in the immunopathogenesis of eczema and therefore could be considered as a potential therapeutic target.

Angelini, F., Di Matteo, G., Balestrero, S., Brunetti, E., Mancino, G., Rossi, P., et al. (2007). Nuclear factor kappa B activity is increased in peripheral blood mononuclear cells of children affected by atopic and non-atopic eczema. INTERNATIONAL JOURNAL OF IMMUNOPATHOLOGY AND PHARMACOLOGY, 20(1), 59-67.

Nuclear factor kappa B activity is increased in peripheral blood mononuclear cells of children affected by atopic and non-atopic eczema

ANGELINI, FEDERICA;ROSSI, PAOLO;
2007-06-01

Abstract

Atopic and non-atopic eczema is an inflammatory cutaneous disease which is common in childhood and is associated with a dysregulation of the immune system. Many genes encoding immune receptors, cytokines, chemokines, chemokine receptors, and adhesion molecules involved in the development of the disease are under the control of transcription factors belonging to the nuclear factor (NF)-kappa B family. To investigate the role of NF-kappa B in the development of eczema, 20 children, affected by relapsing chronic eczema, were enrolled in this study. Eleven of the 20 children showed IgE immunoreactivity and had a positive prick test. The DNA binding activity of NF-kappa B in nuclear extracts of the patients' peripheral blood mononuclear cells (PBMC) was examined by electrophoretic mobility shift assay. We found that basal NF-kappa B-DNA binding activity in PBMC was significantly higher in the eczema patient group in comparison with the same parameter in the healthy age-matched control group. Moreover, we observed a significant correlation between NF-kappa B-DNA binding activity and patients' clinical score (SCORAD). Based on these observations we speculate that NF-kappa B can play an important role in the immunopathogenesis of eczema and therefore could be considered as a potential therapeutic target.
giu-2007
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore MED/38 - PEDIATRIA GENERALE E SPECIALISTICA
English
Allergy; Atopic dermatitis; NF-κB
DNA binding protein; immunoglobulin E; immunoglobulin enhancer binding protein; methylprednisolone; antiinflammatory agent; cytokine; DNA; tumor necrosis factor alpha; article; atopic dermatitis; child; childhood disease; clinical article; clinical feature; controlled study; disease severity; DNA binding; eczema; enzyme inhibition; female; gel mobility shift assay; gene overexpression; human; immune deficiency; immunoglobulin blood level; immunopathology; immunoreactivity; male; priority journal; protein analysis; protein expression; scoring system; biosynthesis; blood; cell nucleus; chemistry; infant; intravenous drug administration; metabolism; monocyte; pathology; preschool child; skin test; Anti-Inflammatory Agents; Cell Nucleus; Child; Child, Preschool; Cytokines; Dermatitis, Atopic; DNA; Eczema; Electrophoretic Mobility Shift Assay; Female; Humans; Immunoglobulin E; Infant; Injections, Intravenous; Male; Methylprednisolone; Monocytes; NF-kappa B; Skin Tests; Tumor Necrosis Factor-alpha
Angelini, F., Di Matteo, G., Balestrero, S., Brunetti, E., Mancino, G., Rossi, P., et al. (2007). Nuclear factor kappa B activity is increased in peripheral blood mononuclear cells of children affected by atopic and non-atopic eczema. INTERNATIONAL JOURNAL OF IMMUNOPATHOLOGY AND PHARMACOLOGY, 20(1), 59-67.
Angelini, F; Di Matteo, G; Balestrero, S; Brunetti, E; Mancino, G; Rossi, P; Galli, E
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/57260
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