Phytosterols (beta-sitosterol, cholestanol and campesterol) and cholesterol precursors (desmosterol and lathosterol), have been suggested as important biochemical markers of intestinal cholesterol absorption and liver biosynthesis, respectively, and as useful clinical parameters in the study of hypercholesterolemia, beta-sitosterolemia, atherosclerosis and cardiovascular disease, including pharmacological response to hypolipidemic agents. We developed an optimised analytical method for the simultaneous analysis,of cholestanol, desmosterol, lathosterol, campesterol and beta-sitosterol in plasma using capillary gas chromatography coupled to mass spectrometry (GC-MS) with multiple selected ion monitoring (SIM). This method is based on the alkaline hydrolysis of sterol esters, extraction of free sterols and derivatization. The recovery of all sterols was in the range 76-101%. Within-day relative standard deviations (R.S.Ds.) and the between-day R.S.Ds. of cholestanol, desmosterol, lathosterol, campesterol and beta-sitosterol were less than 8%, and their plasma levels in 161 normal subjects were (mean +/- S.D.) 4.73 +/- 2.57, 2.37 +/- 1.04, 6.23 +/- 3.14, 3.67 +/- 1.95 and 5.92 +/- 3.62 mu mol/l, respectively. (c) 2006 Elsevier B.V. All-rights reserved.

Ahmida, H., Bertucci, P., Franzo, L., Massoud, R., Cortese, C., Lala, A., et al. (2006). Simultaneous determination of plasmatic phytosterols and cholesterol precursors using gas chromatography-mass spectrometry (GC-MS) with selective ion monitoring (SIM). JOURNAL OF CHROMATOGRAPHY. B, 842(1), 43-47 [10.1016/j.jchromb.2006.05.024].

Simultaneous determination of plasmatic phytosterols and cholesterol precursors using gas chromatography-mass spectrometry (GC-MS) with selective ion monitoring (SIM)

MASSOUD, RENATO;CORTESE, CLAUDIO;LALA, ALBERTO;FEDERICI, GIORGIO
2006-01-01

Abstract

Phytosterols (beta-sitosterol, cholestanol and campesterol) and cholesterol precursors (desmosterol and lathosterol), have been suggested as important biochemical markers of intestinal cholesterol absorption and liver biosynthesis, respectively, and as useful clinical parameters in the study of hypercholesterolemia, beta-sitosterolemia, atherosclerosis and cardiovascular disease, including pharmacological response to hypolipidemic agents. We developed an optimised analytical method for the simultaneous analysis,of cholestanol, desmosterol, lathosterol, campesterol and beta-sitosterol in plasma using capillary gas chromatography coupled to mass spectrometry (GC-MS) with multiple selected ion monitoring (SIM). This method is based on the alkaline hydrolysis of sterol esters, extraction of free sterols and derivatization. The recovery of all sterols was in the range 76-101%. Within-day relative standard deviations (R.S.Ds.) and the between-day R.S.Ds. of cholestanol, desmosterol, lathosterol, campesterol and beta-sitosterol were less than 8%, and their plasma levels in 161 normal subjects were (mean +/- S.D.) 4.73 +/- 2.57, 2.37 +/- 1.04, 6.23 +/- 3.14, 3.67 +/- 1.95 and 5.92 +/- 3.62 mu mol/l, respectively. (c) 2006 Elsevier B.V. All-rights reserved.
2006
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore BIO/12 - BIOCHIMICA CLINICA E BIOLOGIA MOLECOLARE CLINICA
English
Con Impact Factor ISI
Cholesterol precursors; Gas chromatography-mass spectrometry; Phytosterols
Absorption; Biosynthesis; Cardiovascular system; Diseases; Gas chromatography; Mass spectrometry; Monitoring; Atherosclerosis; Cardiovascular diseases; Cholesterol precursors; Gas chromatography-mass spectrometry; Phytosterols; Cholesterol; campesterol; cholestanol; cholesterol; cholesterol esterase; desmosterol; hydrolase; lathosterol; phytosterol; sitosterol; sterol; analytic method; article; capillary gas chromatography; controlled study; derivatization; extraction; human; ion monitoring; lipid analysis; lipid blood level; mass spectrometry; normal human; plasma; priority journal; Cholesterol; Gas Chromatography-Mass Spectrometry; Humans; Phytosterols; Reference Standards; Reproducibility of Results; Sensitivity and Specificity
Ahmida, H., Bertucci, P., Franzo, L., Massoud, R., Cortese, C., Lala, A., et al. (2006). Simultaneous determination of plasmatic phytosterols and cholesterol precursors using gas chromatography-mass spectrometry (GC-MS) with selective ion monitoring (SIM). JOURNAL OF CHROMATOGRAPHY. B, 842(1), 43-47 [10.1016/j.jchromb.2006.05.024].
Ahmida, H; Bertucci, P; Franzo, L; Massoud, R; Cortese, C; Lala, A; Federici, G
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/57138
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