Che-1 is a recently identified human Rb binding protein that inhibits the Rb growth-suppressing function and regulates cell proliferation. Che-1 contacts the Rb and competes with HDAC I for Rb-binding site, removing HDAC I from the Rb/E2F cell cycle-regulated promoters. We have investigated the expression of Che-1 in neuronal cells and we showed that Che-1 directly interacts with Tau. Tau is a microtubule-associated protein involved in the assembly and stabilization of neuronal microtubules network that plays a crucial role modulating neuronal morphogenesis, axonal shape, and transport. In rat cerebellar granule neurons (CGNs) Che-1 partially colocalizes with Tau in the cytoplasm. Che-1 binds the amino-terminal region of Tau protein, which is not involved in microtubule interactions. Tau and Che-1 endogenous proteins coimmunoprecipitate from CGNs cellular lysates. In addition, Che-1/Tau interaction was demonstrated both in overexpressing COS-7 cells and CGNs by FRET analysis. Finally, we observed that Tau/Che-1 interaction is modulated during neuronal apoptosis. (C) 2003 Elsevier Inc. All rights reserved.

Barbato, C., Corbi, N., Canu, N., Fanciulli, M., Serafino, A., Ciotti, M., et al. (2003). Rb binding protein Che-1 interacts with Tau in cerebellar granule neurons: Modulation during neuronal apoptosis. MOLECULAR AND CELLULAR NEUROSCIENCES, 24(4), 1038-1050 [10.1016/j.mcn.2003.08.002].

Rb binding protein Che-1 interacts with Tau in cerebellar granule neurons: Modulation during neuronal apoptosis

CANU, NADIA;CALISSANO, PIETRO;
2003

Abstract

Che-1 is a recently identified human Rb binding protein that inhibits the Rb growth-suppressing function and regulates cell proliferation. Che-1 contacts the Rb and competes with HDAC I for Rb-binding site, removing HDAC I from the Rb/E2F cell cycle-regulated promoters. We have investigated the expression of Che-1 in neuronal cells and we showed that Che-1 directly interacts with Tau. Tau is a microtubule-associated protein involved in the assembly and stabilization of neuronal microtubules network that plays a crucial role modulating neuronal morphogenesis, axonal shape, and transport. In rat cerebellar granule neurons (CGNs) Che-1 partially colocalizes with Tau in the cytoplasm. Che-1 binds the amino-terminal region of Tau protein, which is not involved in microtubule interactions. Tau and Che-1 endogenous proteins coimmunoprecipitate from CGNs cellular lysates. In addition, Che-1/Tau interaction was demonstrated both in overexpressing COS-7 cells and CGNs by FRET analysis. Finally, we observed that Tau/Che-1 interaction is modulated during neuronal apoptosis. (C) 2003 Elsevier Inc. All rights reserved.
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore BIO/09
English
Con Impact Factor ISI
binding protein; CheA kinase; tau protein; apoptosis; article; cell cycle; cell line; cell lysate; cell structure; cerebellum; cytoplasm; human; human cell; immunoprecipitation; microtubule; morphogenesis; nerve cell; nerve fiber; priority journal; protein analysis; protein assembly; protein binding; protein expression; protein function; protein interaction; protein localization; protein transport; Animals; Apoptosis; Cercopithecus aethiops; Cerebellum; Dogs; Humans; Mutation; Neurons; Protein Binding; Protein Kinases; Rats; Rats, Wistar; Retinoblastoma Protein; tau Proteins
Barbato, C., Corbi, N., Canu, N., Fanciulli, M., Serafino, A., Ciotti, M., et al. (2003). Rb binding protein Che-1 interacts with Tau in cerebellar granule neurons: Modulation during neuronal apoptosis. MOLECULAR AND CELLULAR NEUROSCIENCES, 24(4), 1038-1050 [10.1016/j.mcn.2003.08.002].
Barbato, C; Corbi, N; Canu, N; Fanciulli, M; Serafino, A; Ciotti, M; Libri, V; Bruno, T; Amadoro, G; De Angelis, R; Calissano, P; Passananti, C
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2108/56917
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