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EnglishRecent developments in single molecule force spectroscopy have allowed investigating the interaction between two redox partners, Azurin and Cytochrome C 551. Azurin has been directly chemisorbed on a gold electrode whereas cytochrome c has been linked to the atomic force microscopy tip by means of a heterobifunctional flexible cross-linker. When recording force-distance cycles, molecular recognition events could be observed, displaying unbinding forces of ∼95 pN for an applied loading rate of 10 nN/s. The specificity of molecular recognition was confirmed by the significant decrease of unbinding probability observed in control block experiments performed adding free azurin solution in the fluid cell. In addition, the complex dissociation kinetics has been here investigated by monitoring the unbinding forces as a function of the loading rate: the thermal off-rate was estimated to be ∼14 s−1, much higher than values commonly estimated for complexes more stable than electron transfer complexes. Results here discussed represent the first studies on molecular recognition between two redox partners by atomic force microscopy.
Bonanni, B., Kamruzzahan, A., Bizzarri, A.R., Rankl, C., Gruber, H.J., Hinterdorfer, P., et al. (2005). Single Molecule Recognition between Cytochrome C 551 and Gold-Immobilized Azurin by Force Spectroscopy. BIOPHYSICAL JOURNAL, 89(4), 2783-2791.
| Tipologia: | Articolo su rivista |
| Citazione: | Bonanni, B., Kamruzzahan, A., Bizzarri, A.R., Rankl, C., Gruber, H.J., Hinterdorfer, P., et al. (2005). Single Molecule Recognition between Cytochrome C 551 and Gold-Immobilized Azurin by Force Spectroscopy. BIOPHYSICAL JOURNAL, 89(4), 2783-2791. |
| IF: | Con Impact Factor ISI |
| Lingua: | English |
| Settore Scientifico Disciplinare: | Settore FIS/03 - Fisica della Materia |
| Revisione (peer review): | Sì, ma tipo non specificato |
| Tipo: | Articolo |
| Rilevanza: | Rilevanza internazionale |
| Digital Object Identifier (DOI): | http://dx.doi.org/10.1529/biophysj.105.064097 |
| Stato di pubblicazione: | Pubblicato |
| Data di pubblicazione: | ott-2005 |
| Titolo: | Single Molecule Recognition between Cytochrome C 551 and Gold-Immobilized Azurin by Force Spectroscopy |
| Autori: | |
| Autori: | Bonanni, B; Kamruzzahan, ASM; Bizzarri, AR; Rankl, C; Gruber, HJ; Hinterdorfer, P; Cannistraro, S |
| Appare nelle tipologie: | 01 - Articolo su rivista |
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