The aim of our study is to assess the mortality of leukocytes during extracorporeal photopheresis. Sixty-three photopheresis performed on 13 patients affected by chronic GvHD were evaluated. Samples were analyzed using a FACSCalibur flow cytometer. Apoptosis and necrosis of limphomononuclear cells dramatically increased after the apheretic procedure. We found a further increase of apoptotic and necrotic limphomononuclear cells after treatment with 8-MOP and UVA (p⩽0.05). Our data suggested that the immunomodulatory effects of extracorporeal photopheresis, triggered by circulating apoptotic or necrotic cells, could play an important role in the treatment of GvHD with this procedure.

Daniele, N., Del Proposto, G., Cerrone, P., Sinopoli, S., Sansone, L., Gadaleta, D., et al. (2012). Evaluation of cell death after treatment with extracorporeal photopheresis. TRANSFUSION AND APHERESIS SCIENCE, 46(1), 53-57 [10.1016/j.transci.2011.11.002].

Evaluation of cell death after treatment with extracorporeal photopheresis

ZINNO, FRANCESCO;ADORNO, GASPARE;ISACCHI, GIANCARLO
2012-02-01

Abstract

The aim of our study is to assess the mortality of leukocytes during extracorporeal photopheresis. Sixty-three photopheresis performed on 13 patients affected by chronic GvHD were evaluated. Samples were analyzed using a FACSCalibur flow cytometer. Apoptosis and necrosis of limphomononuclear cells dramatically increased after the apheretic procedure. We found a further increase of apoptotic and necrotic limphomononuclear cells after treatment with 8-MOP and UVA (p⩽0.05). Our data suggested that the immunomodulatory effects of extracorporeal photopheresis, triggered by circulating apoptotic or necrotic cells, could play an important role in the treatment of GvHD with this procedure.
feb-2012
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore MED/05 - PATOLOGIA CLINICA
English
Con Impact Factor ISI
Daniele, N., Del Proposto, G., Cerrone, P., Sinopoli, S., Sansone, L., Gadaleta, D., et al. (2012). Evaluation of cell death after treatment with extracorporeal photopheresis. TRANSFUSION AND APHERESIS SCIENCE, 46(1), 53-57 [10.1016/j.transci.2011.11.002].
Daniele, N; Del Proposto, G; Cerrone, P; Sinopoli, S; Sansone, L; Gadaleta, D; Lanti, A; Ferraro, A; Spurio, S; Scerpa, M; Zinno, F; Adorno, G; Isacchi, G
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/56224
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