The active-site copper ion of the prokaryotic Cu,Zn superoxide dismutase from P. leiognathi is found to undergo reversible reduction upon irradiation of the protein solution with a high-intensity X-ray beam from a third-generation synchrotron source. The same phenomenon is observed for the enzyme crystals, whose diffraction pattern has been obtained from synchrotron sources. In this case the active-site copper-ligand coordination bond lengths and in particular the Cu-NE2(His61) distance are consistent with a copper ion in the reduced state. These results are in line with previous studies on the eukaryotic Cu,Zn superoxide dismutases and suggest the conservation of an identical catalytic mechanism in both the prokaryotic and eukaryotic enzymes. (C) 1998 Academic Press.

Stroppolo, M., Nuzzo, S., Pesce, A., Rosano, C., Battistoni, A., Bolognesi, M., et al. (1998). On the coordination and oxidation states of the active-site copper ion in prokaryotic Cu,Zn superoxide dismutases. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 249(3), 579-582.

On the coordination and oxidation states of the active-site copper ion in prokaryotic Cu,Zn superoxide dismutases

BATTISTONI, ANDREA;DESIDERI, ALESSANDRO
1998-01-01

Abstract

The active-site copper ion of the prokaryotic Cu,Zn superoxide dismutase from P. leiognathi is found to undergo reversible reduction upon irradiation of the protein solution with a high-intensity X-ray beam from a third-generation synchrotron source. The same phenomenon is observed for the enzyme crystals, whose diffraction pattern has been obtained from synchrotron sources. In this case the active-site copper-ligand coordination bond lengths and in particular the Cu-NE2(His61) distance are consistent with a copper ion in the reduced state. These results are in line with previous studies on the eukaryotic Cu,Zn superoxide dismutases and suggest the conservation of an identical catalytic mechanism in both the prokaryotic and eukaryotic enzymes. (C) 1998 Academic Press.
1998
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore BIO/10 - BIOCHIMICA
English
Con Impact Factor ISI
bacterial Cu,Zn superoxide dismutase; X-ray diffraction; XANES; copper coordination redox state
4
Stroppolo, M., Nuzzo, S., Pesce, A., Rosano, C., Battistoni, A., Bolognesi, M., et al. (1998). On the coordination and oxidation states of the active-site copper ion in prokaryotic Cu,Zn superoxide dismutases. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 249(3), 579-582.
Stroppolo, M; Nuzzo, S; Pesce, A; Rosano, C; Battistoni, A; Bolognesi, M; Mobilio, S; Desideri, A
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/55143
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