The catalytic activity of a mutant of Photobacterium leiognathi Cu,Zn superoxide dismutase in which the Glu59 residue, conserved in most bacterial variants of the enzyme, has been replaced by glutamine was investigated by pulse radiolysis. At neutral pH the enzyme was found to have a k(cat)/K(M) of 1.0 Â± 0.1 x 1010 M-1 s-1 the highest value ever found for any superoxide dismutase. Brownian dynamics simulation suggests that such a high value is due to an enhanced substrate attraction by the modified electric field distribution. The mutant is also characterized by an active-site widely accessible for the solvent, since iodide is able to interact with the copper atom with an affinity constant twice as high as that found in the native enzyme. The large solvent accessible surface of the copper site together with a favorable distribution of the protein-generated electric field gives rise to the most efficient enzyme ever found with activity close to the diffusion limit.
Folcarelli, S., Venerini, F., Battistoni, A., O'Neill, P., Rotilio, G., & Desideri, A. (1999). Toward the engineering of a super efficient enzyme. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 256(2), 425-428.
|Tipologia:||Articolo su rivista|
|Citazione:||Folcarelli, S., Venerini, F., Battistoni, A., O'Neill, P., Rotilio, G., & Desideri, A. (1999). Toward the engineering of a super efficient enzyme. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 256(2), 425-428.|
|IF:||Con Impact Factor ISI|
|Settore Scientifico Disciplinare:||Settore BIO/10|
|Revisione (peer review):||Sì, ma tipo non specificato|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1006/bbrc.1999.0211|
|Stato di pubblicazione:||Pubblicato|
|Data di pubblicazione:||1999|
|Titolo:||Toward the engineering of a super efficient enzyme|
|Autori:||Folcarelli, S; Venerini, F; Battistoni, A; O'Neill, P; Rotilio, G; Desideri, A|
|Appare nelle tipologie:||01 - Articolo su rivista|