Lys-120 and Lys-134, located at the edge of the active site channel in most Cu,Zn superoxide dismutases, have been suggested to play a major role in steering the anionic substrate towards the catalytic copper ion. In this study, mutants of Xenopus laevis Cu,Zn superoxide dismutase have been engineered, with Lys-120 and Lys-134 changed into leucine and threonine, respectively, and their catalytic properties have been investigated by pulse radiolysis. Results obtained demonstrate that both residues decrease the catalytic rate by about 40%, in partial disagreement with previous brownian dynamics calculations, carried out on bovine Cu,Zn superoxide dismutase.

Polticelli, F., Battistoni, A., Bottaro, G., Carri', M.t., O'Neill, P., Desideri, A., et al. (1994). Mutation of Lys-120 and Lys-134 drastically reduces the catalytic rate of Cu,Zn superoxide dismutase. FEBS LETTERS, 352(1), 76-78 [10.1016/0014-5793(94)00885-X].

Mutation of Lys-120 and Lys-134 drastically reduces the catalytic rate of Cu,Zn superoxide dismutase

BATTISTONI, ANDREA;CARRI', MARIA TERESA;DESIDERI, ALESSANDRO;ROTILIO, GIUSEPPE
1994-01-01

Abstract

Lys-120 and Lys-134, located at the edge of the active site channel in most Cu,Zn superoxide dismutases, have been suggested to play a major role in steering the anionic substrate towards the catalytic copper ion. In this study, mutants of Xenopus laevis Cu,Zn superoxide dismutase have been engineered, with Lys-120 and Lys-134 changed into leucine and threonine, respectively, and their catalytic properties have been investigated by pulse radiolysis. Results obtained demonstrate that both residues decrease the catalytic rate by about 40%, in partial disagreement with previous brownian dynamics calculations, carried out on bovine Cu,Zn superoxide dismutase.
1994
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore BIO/10 - BIOCHIMICA
English
Con Impact Factor ISI
SUPEROXIDE DISMUTASE; SITE-DIRECTED MUTAGENESIS; PULSE RADIOLYSIS; BROWNIAN DYNAMICS; ELECTROSTATIC INTERACTION
Polticelli, F., Battistoni, A., Bottaro, G., Carri', M.t., O'Neill, P., Desideri, A., et al. (1994). Mutation of Lys-120 and Lys-134 drastically reduces the catalytic rate of Cu,Zn superoxide dismutase. FEBS LETTERS, 352(1), 76-78 [10.1016/0014-5793(94)00885-X].
Polticelli, F; Battistoni, A; Bottaro, G; Carri', Mt; O'Neill, P; Desideri, A; Rotilio, G
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/55132
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