Over-expression of the chaperonins GroEL and GroES significantly suppressed the temperature-dependent pattern of expression of Cu,Zn superoxide dismutases in Escherichia coli and increased the yield of active enzyme. The results obtained indicate that chaperonins prevent degradation of metal-deficient enzyme molecules. GroEL was shown to form a complex with unfolded Cu,Zn superoxide dismutase in vitro, confirming that GroEL can interact with beta-stranded proteins.
Battistoni, A., Carri', M.t., Steinkuhler, C., Rotilio, G. (1993). Chaperonins dependent increase of Cu,Zn superoxide dismutase production in Escherichia coli. FEBS LETTERS, 322(1), 6-9 [10.1016/0014-5793(93)81099-L].
Chaperonins dependent increase of Cu,Zn superoxide dismutase production in Escherichia coli
BATTISTONI, ANDREA;CARRI', MARIA TERESA;ROTILIO, GIUSEPPE
1993-01-01
Abstract
Over-expression of the chaperonins GroEL and GroES significantly suppressed the temperature-dependent pattern of expression of Cu,Zn superoxide dismutases in Escherichia coli and increased the yield of active enzyme. The results obtained indicate that chaperonins prevent degradation of metal-deficient enzyme molecules. GroEL was shown to form a complex with unfolded Cu,Zn superoxide dismutase in vitro, confirming that GroEL can interact with beta-stranded proteins.| File | Dimensione | Formato | |
|---|---|---|---|
|
febs 1993.pdf
solo utenti autorizzati
Licenza:
Creative commons
Dimensione
379.48 kB
Formato
Adobe PDF
|
379.48 kB | Adobe PDF | Visualizza/Apri Richiedi una copia |
Questo articolo è pubblicato sotto una Licenza Licenza Creative Commons
