The thermal stability of two single (K3R, K67R) and one double (K3R-K67R) mutants of Xenopus laevis B Cu,Zn superoxide dismutase has been studied to test Lys-->Arg substitution as an 'electrostatically conservative' strategy to increase protein stability, The K3R mutant displays an increased thermostability with respect to the wild-type enzyme, whilst a decreased stability was observed in the case of the K67R and K3R-K67R mutants, Concentration dependence of the apparent inactivation constant (k(app)) of the latter mutants, as compared to that of the wild type enzyme and K3R mutant, indicates that their higher sensitivity to heat inactivation is due to a perturbation of the dimer association. These results are confirmed also by fluorescence anisotropy measurements of the internal probe Tyr149. The possible role of Arg67 in perturbing the dimer dissociation equilibrium toward the monomeric form is discussed.
Folcarelli, S., Battistoni, A., Carri', M.t., Polticelli, F., Falconi, M., Nicolini, L., et al. (1996). Effect of Lys->Arg mutation on the thermal stability of Cu,Zn superoxide dismutase: Influence on the monomer-dimer equilibrium. PROTEIN ENGINEERING, 9(4), 323-325.
Tipologia: | Articolo su rivista |
Citazione: | Folcarelli, S., Battistoni, A., Carri', M.t., Polticelli, F., Falconi, M., Nicolini, L., et al. (1996). Effect of Lys->Arg mutation on the thermal stability of Cu,Zn superoxide dismutase: Influence on the monomer-dimer equilibrium. PROTEIN ENGINEERING, 9(4), 323-325. |
IF: | Con Impact Factor ISI |
Lingua: | English |
Settore Scientifico Disciplinare: | Settore BIO/10 |
Revisione (peer review): | Sì, ma tipo non specificato |
Tipo: | Articolo |
Rilevanza: | Rilevanza internazionale |
Stato di pubblicazione: | Pubblicato |
Data di pubblicazione: | 1996 |
Titolo: | Effect of Lys->Arg mutation on the thermal stability of Cu,Zn superoxide dismutase: Influence on the monomer-dimer equilibrium |
Autori: | |
Autori: | Folcarelli, S; Battistoni, A; Carri', Mt; Polticelli, F; Falconi, M; Nicolini, L; Stella, L; Rosato, N; Rotilio, G; Desideri, A |
Appare nelle tipologie: | 01 - Articolo su rivista |
File in questo prodotto:
File | Descrizione | Tipologia | Licenza | |
---|---|---|---|---|
Protein Engineering.pdf | N/A | Copyright dell'editore | Administrator Richiedi una copia |