The functional properties of hemoglobins from Triturus cristatus carnifex have been characterized both from an equilibrium and a kinetic point of view. A special attention has been given to the effect of organic phosphates in view of their role in the modulation of the hemoglobin function. Under stripped conditions newt hemoglobins display a negative Bohr effect which becomes much less pronounced in the presence of 0.2 M inorganic phosphates. The effect of organic phosphates involves not only a drop in the O2 affinity but also a large change in the shape of the O2 Bohr effect which becomes markedly positive in the presence of myo-inositol hexakis(phosphate). The experimental data reported make it possible to give a complete picture of the functional behaviour of newt hemoglobins, making a contribution to clarifying the dependence of the sign of the Bohr effect on the acclimatization temperature of the animals that has been previously reported [Morpurgo, G., Battaglia, P. A. and Leggio, T. (1970) Nature (Lond.) 225, 76--77].

Condo', S.g., Giardina, B., Lunadei, M., Ferracin, A., Brunori, M. (1981). Functional properties of hemoglobins from Triturus cristatus. EUROPEAN JOURNAL OF BIOCHEMISTRY, 120(2), 323-327.

Functional properties of hemoglobins from Triturus cristatus

CONDO', SAVERIO GIOVANNI;
1981-11-01

Abstract

The functional properties of hemoglobins from Triturus cristatus carnifex have been characterized both from an equilibrium and a kinetic point of view. A special attention has been given to the effect of organic phosphates in view of their role in the modulation of the hemoglobin function. Under stripped conditions newt hemoglobins display a negative Bohr effect which becomes much less pronounced in the presence of 0.2 M inorganic phosphates. The effect of organic phosphates involves not only a drop in the O2 affinity but also a large change in the shape of the O2 Bohr effect which becomes markedly positive in the presence of myo-inositol hexakis(phosphate). The experimental data reported make it possible to give a complete picture of the functional behaviour of newt hemoglobins, making a contribution to clarifying the dependence of the sign of the Bohr effect on the acclimatization temperature of the animals that has been previously reported [Morpurgo, G., Battaglia, P. A. and Leggio, T. (1970) Nature (Lond.) 225, 76--77].
nov-1981
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore BIO/10 - BIOCHIMICA
English
Oxygen; Animals; Hemoglobins; Triturus; Kinetics; Hydrogen-Ion Concentration; Erythrocytes; Carbon Monoxide; Adenosine Triphosphate
Condo', S.g., Giardina, B., Lunadei, M., Ferracin, A., Brunori, M. (1981). Functional properties of hemoglobins from Triturus cristatus. EUROPEAN JOURNAL OF BIOCHEMISTRY, 120(2), 323-327.
Condo', Sg; Giardina, B; Lunadei, M; Ferracin, A; Brunori, M
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/54863
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