The incorporation and redistribution of [1-14C]arachidonic acid in SK-N-BE human neuroblastoma cell phospholipids were investigated. By continuous labelling in serum-enriched medium, a rapid radioactivity incorporation into phosphatidylcholine (PtdCho), phosphatidylinositol, and phosphatidylserine was observed; initially, phosphatidylethanolamine (PtdEtn) was poorly labelled, but at later stages it displayed the highest level of arachidonic acid incorporation, in comparison with other phospholipid classes. Labelling of triacylglycerols was also observed. When cells were pulse-labelled with [1-14C]arachidonic acid and then reincubated in label-free medium, a decrease of the radioactivity in triacylglycerols was observed initially, paralleled by an increase of phospholipid labelling; thereafter, arachidonic acid redistribution was consistent with a net decrease of the radioactivity associated with PtdCho acid-stable forms (i.e., diacyl plus alkylacyl forms), concomitantly with a net labelling increase of both acid-stable PtdEtn and alkenylacyl-PtdEtn. Data indicate the following: (a) neuroblastoma cells incorporate arachidonic acid into phospholipids through complex kinetics involving transfer of the fatty acid from acid-stable PtdCho to both alkenylacyl-PtdEtn and acid-stable PtdEtn; and (b) triacylglycerols act as storage molecules for arachidonic acid which is subsequently incorporated into phospholipids. The possibility that arachidonic acid transfer to PtdEtn subclasses is driven by distinct mechanisms is discussed.
Spinedi A., P.L. (1990). Arachidonic acid incorporation and redistribution in human neuroblastoma (SK-N-BE) cell phospholipids. JOURNAL OF NEUROCHEMISTRY, 54(3), 778-782.
|Tipologia:||Articolo su rivista|
|Citazione:||Spinedi A., P.L. (1990). Arachidonic acid incorporation and redistribution in human neuroblastoma (SK-N-BE) cell phospholipids. JOURNAL OF NEUROCHEMISTRY, 54(3), 778-782.|
|IF:||Con Impact Factor ISI|
|Settore Scientifico Disciplinare:||Settore BIO/09|
|Revisione (peer review):||Sì, ma tipo non specificato|
|Stato di pubblicazione:||Pubblicato|
|Data di pubblicazione:||1990|
|Titolo:||Arachidonic acid incorporation and redistribution in human neuroblastoma (SK-N-BE) cell phospholipids|
|Autori:||Spinedi A., Pacini L., Piacentini M., Melino G., Luly P.|
|Appare nelle tipologie:||01 - Articolo su rivista|