The temperature-dependence of both the lipid order parameter (S(DPH)) and acetylcholinesterase (AChE) activity from native and cholesterol-enriched human erythrocyte membranes was investigated. Cholesterol enrichment abolishes an inflection observed around 30°C in the temperature-dependence of native membrane lipid order parameter, whereas the Arrhenius plot of the enzymic activity is substantially unaffected. These results support the view that the breaks in the Arrhenius plot of the enzyme activity are not related to sudden changes of bulk membrane physical state, but arise from a direct effect of temperature on enzyme conformation.
Spinedi, A., Rufini, S., Luly, P., & Farias, F.N. (1988). The temperature-dependence of human erythrocyte acetylcholinesterase activity is not affected by membrane cholesterol enrichment. BIOCHEMICAL JOURNAL, 255(2), 547-551.
Tipologia: | Articolo su rivista |
Citazione: | Spinedi, A., Rufini, S., Luly, P., & Farias, F.N. (1988). The temperature-dependence of human erythrocyte acetylcholinesterase activity is not affected by membrane cholesterol enrichment. BIOCHEMICAL JOURNAL, 255(2), 547-551. |
IF: | Con Impact Factor ISI |
Lingua: | English |
Settore Scientifico Disciplinare: | Settore BIO/09 |
Revisione (peer review): | Sì, ma tipo non specificato |
Tipo: | Articolo |
Rilevanza: | Rilevanza internazionale |
Stato di pubblicazione: | Pubblicato |
Data di pubblicazione: | 1988 |
Titolo: | The temperature-dependence of human erythrocyte acetylcholinesterase activity is not affected by membrane cholesterol enrichment |
Autori: | |
Autori: | Spinedi, A; Rufini, S; Luly, P; Farias, FN |
Appare nelle tipologie: | 01 - Articolo su rivista |